YZ XZ XY Fig. Supp 1: PSF for BioRad MRC-1024 laser-scanning confocal microscope with 60x oil immersion lens (NA 1.4). The PSF was sampled with a resolution.

Slides:



Advertisements
Similar presentations
Imaging early embryos and stem cells Centre for Trophoblast Research Anne McLaren Laboratory for Regenerative Medicine University of Cambridge Kathy Niakan.
Advertisements

Nick Beavers Project Manager Deconvolution from Andy Molnar Software Engineer.
1. Resolution (R) : separation of close objects, light wavelength NA, numerical aperture 2. Contrast : distinction of objects from background “light field”
Scanning Confocal Basics n What’s so special ? n Confocals give higher resolution than normal microscopes. n Confocals can OPTICALLY SECTION a sample.
Dave Piston May 18, 2014 Optical Sectioning 2: Confocal Designs and “Pseudo-confocals” Outline 1.PMT detectors 2.Laser Scanning Design Features 3.Pseudo-confocals.
Image-Pro Premier Basic Training Course
Comparison and Implications of Three Optical Microscopy Data Acquisition Modalities James Butler Ph.D. Nikon Instruments, Inc. Widefield Fluorescence Confocal.
Confocal Laser Microscopy at the MHH. Laser Microscopy Facility Zentrale Forschungseinrichtung Lasermikroskopie Location: Building I4, Level 01 Contact:
A) 80 b) 53 c) 13 d) x 2 = : 10 = 3, x 3 = 309.
Resolution ä What can be expected ? ä What affects it ? ä How can it be improved ?
Gate-level Design: Full Adder  Truth table: Note: Z - carry in (to the current position) C - carry out (to the next position)  Using K-map, simplified.
Multiplexer as a Universal Function Generator
Reflexive -- First sentence of proof is: (1) Let x  Z (2) Let (x,x)  R. (3) Let (x,x)  I (4) Let x  R.
Microscopy Techniques for Biomaterial Characterization: A Primer Prabhas V. Moghe Lecture 3 September 21, 1999 RU CBE 533 or BME 553; NJIT BME 698.
Figure 1.1 The observer in the truck sees the ball move in a vertical path when thrown upward. (b) The Earth observer views the path of the ball as a parabola.
Introduction to Microscopy. Objectives Learn to use a compound microscope correctly. Diagram the path of light through a compound microscope. Name major.
1.4: Rewrite Formulas & Equations
A microscope is a Complicated Lens n The microscope can be represented by a single convex lens.
Bioimaging ChemEng 590B: Lecture 15 4/11/13. Rat mammary carcinoma cells 10 min, images every 20 seconds Michele Balsamo, Gertler lab MIT 1. Imaging Cells.
Bioimaging ChemEng 575: Lecture 16 4/15/14. Rat mammary carcinoma cells 10 min, images every 20 seconds Michele Balsamo, Gertler lab MIT 1. Imaging Cells.
MAGNIFICATION versus RESOLUTION of a Microscope GEOL 3213, Micropaleontology.
GSCAM5 and Olympus Inverted Microscope with x10 objective lens field of view: width: mm field of view: height: mm.
IPC Friedrich-Schiller-Universität Jena 1 Reduction of out of focus light  Excitation light excites fluorescence more or less within the whole sample.
Tile Scanning on Zeiss Microscopes Kim Peifley 2/09/15.
Factoring By Grouping Students will be able to factor polynomials by grouping.
Basics of Deconvolution
Microscopes Compound Bright-Field Light Microscope
Arbitrary and Dynamic Patterning in a Programmable Array Microscope
Sneha.  A combinational circuit that performs the addition of two bits is called a half adder.  It has two inputs.  It has two outputs.
MICROSCOPE DESIGN. beam expander and objective Why do we need a beam expander? What facts about the laser do we need to know? How much expansion do we.
Microscope.
5.3 Theorems Involving Parallel Lines
XDI RDF Graphing V x+y $has +x+y $has +x+y +x/$has/+y +x+y +x+y $has +x+y/$has/+z +x/$has/+y+z +x+y+z +x+y+z $has +z +x+y+z $has 3 4 Full.
Topic 1 Microscopes.
Use Snell’s law to determine the angle of total internal reflection in the coverslip (without oil). Oil immersion objectives More light (no total internal.
Principles and Practices of Light Microscopy II: Brightfield optics, resolution, and aberrations.
Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. The schematic of the setup: (a) the multiphoton laser path and (b) the laser path.
Confocal Microscopy Kurt Thorn NIC.
Date of download: 5/30/2016 Copyright © 2016 SPIE. All rights reserved. Working principle of the immersion schemes: (a) focusing in air, (b) focusing through.
Topic 1 Microscopes and Microscopy. Light Microscopes  How do they work?  Optical magnification  Images pass through a lens or a series of lenses 
Starter: Microscopes Which image is from the light microsope? How do you know?
EXAMPLES- Simplifying Radicals part 2
Supplementary Figure S1
Gate-level Design: Full Adder
Drawing Triangles.
Supplemental Figure 5 A B
Types of Microscopes Light Electron Bright field Dark-field Scanning
Response: Movement of mitochondria during CTL-mediated killing
Super-resolution stimulated emission depletion imaging of slit diaphragm proteins in optically cleared kidney tissue  David Unnersjö-Jess, Lena Scott,
James D. Lechleiter, Da-Ting Lin, Ilse Sieneart  Biophysical Journal 
5.1 Midsegments of Triangles
A C B D 1. Read the information about different sorts of microscope.
LIGHT MICROSCOPY basic
Volume 108, Issue 12, Pages (June 2015)
Types of Microscopes Light Electron Bright field Dark-field Scanning
The Microscope.
Image Restoration for Confocal Microscopy: Improving the Limits of Deconvolution, with Application to the Visualization of the Mammalian Hearing Organ 
STRO-1+ mesenchymal precursor cells located in synovial surface projections of patients with osteoarthritis  A. Giurea, M.D., B.M. Rüger, M.M.L.Sc., D.
Factoring Polynomials
C.2.10 Sample Questions.
C.2.8 Sample Questions.
C.2.8 Sample Questions.
Rajendra N. Mitra, Min Zheng, Zongchao Han
3 A Figure 1. Schematic of a conventional scanning tunneling microscope (STM).
NA determines intensity.
The ABCG2-K86M mutant resides in the ER
Volume 85, Issue 6, Pages (December 2003)
The effect of spherical aberrations on images.
Missing values multiplying monomial.
Our microscopes Olympus IX81 (wide-field fluorescence, transmission modes, TIRF) Olympus BF51 (color images, less demanding fluorescence applications)
Presentation transcript:

YZ XZ XY Fig. Supp 1: PSF for BioRad MRC-1024 laser-scanning confocal microscope with 60x oil immersion lens (NA 1.4). The PSF was sampled with a resolution of 0.13 x 0.13 x 0.13 m. The full width at half maximum was 0.31 and 1.05 m in XY and Z-direction, respectively.

YZ XZ XY Fig. Supp 2: PSF for Zeiss LSM confocal microscope with 63x oil immersion lens (NA 1.4). The PSF was sampled with a resolution of 0.1 x 0.1 x 0.1 m. The full width at half maximum was 0.28 and 0.83 m in XY and Z-direction, respectively.