Jeopardy Final Jeopardy Energetics& Enzymes Metabolism Photosynthesis DNA $100 $100 $100 $100 $200 $200 $200 $200 $300 $300 $300 $300 $400 $400 $400 $400 $500 $500 $500 $500 Final Jeopardy

1 - $100 A, anabolic reactions require energy The manufacture of a large protein from hundreds of amino acids is likely to a. require energy input b. release energy that can potentially be used for other purposes c. result in no change in free energy d. be exergonic, with a large negative ΔG A, anabolic reactions require energy

1 - $200 Which of the following statements about enzymes is FALSE? a. Enzyme catalysis is dependent on the three-dimensional structure or conformation of the enzyme. b. Enzymes provide activation energy for the reaction they catalyze. c. Enzymes are composed primarily of protein, but they may bind nonprotein cofactors. d. Enzyme activity can be inhibited by other molecules, even if they do not bind to the active site. B

1 - $300 Why is ATP important for metabolic processes? The breakdown (use of ATP) is an exergonic reaction, releases energy. This energy can be used in endergonic reactions that take in energy.

1 - $400 A long polypeptide is unable to be broken down unless the enzyme protease is added. What is the reason for this? The reaction had too high of activation energy to proceed in the reaction. The enzyme helped to lower the energy barrier to proceed with the reaction.

1 - $500 Based on the following graph: Negative ∆G Exergonic Describe the ∆G Classify as endergonic/exergonic Describe spontaneity Negative ∆G Exergonic Spontaneous

2 - $100 What molecule is produced by glycolysis, and is then imported into the mitochondria to enter into the TCA cycle? pyruvate

2 - $200 Shutdown of an early step in a metabolic pathway by an end product of that pathway is best described, without any additional information, as Feedback inhibition

2 - $300 A proton ionophore is a substance that creates a proton leak in a membrane. What might you predict that a proton ionophore would do in mitochondria? Decrease the amount of ATP produced.

2 - $400 A drug that increases the number of NADH & FADH2 molecules produced through the citric acid cycle/Krebs cycle and has no other effects on the cell would theoretically affect the ATP production in what way? Increase the number of ATP molecules per glucose molecule due to an increase in electrons given to power the ETC, creating a greater H+ gradient, and leading to more ATP produced.

2 - $500 DCA works to increase the production of ATP. Through what mechanism does this occur. It blocks the kinase, which originally works to inactivate PDH. PDH is involved in converting pyruvate to acetyl coA to be used in cellular respiration.

3 - $100 The term kinase means that this protein Adds a phosphate group to the substrate

3 - $200 In a plant cell, where are the ATP synthetase complexes located? Thylakoid membrane: photosynthesis Inner mitochondrial membrane: cellular respiration

3 - $300 A plant is located in an area that has longer nights that it does days, what 2 substances will the plant start to deplete as the night goes on and what 2 substances will it accumulate? ATP and NADPH produced by the light reactions will deplete as its taken up in the calvin cycle. NADP+ and ADP will start to accumulate as the light reactions are unable to take place without light

3 - $400 A plant has difficulty exciting electrons when carrying out photosynthesis, what part of the chloroplast has a defect? Chlorophyll, this pigment harvests light. Located in the thylakoid membranes

3 - $500     Experiment 1. H2O is the final electron acceptor in photosynthesis and produces O2.

4 - $100 How are the 2 strands that make up the DNA double helix held together? Hydrogen bonds between a purine and pyrmidine nitrogenous base.

4 - $200 During translation, what is the direction of polypeptide synthesis? N-terminal to C-terminal

4 - $300 Explain the central dogma theory. DNA is transcribed to create mRNA which is translated to create polypeptides.

4 - $400 Which is the lagging strannd? A

4 - $500 If a purple DNA strand is replicated in a solution of bases that were blue colored, and what would the ratio of purple to blue be in the newly synthesized pieces of DNA? The new strands would contain half purple and half blue strands.

Final Jeopardy Translate this following sequence to reveal the word that corresponds. 5’-AUGCCAAGAGCUUGUACUAUUUGCGAA-3’ Practice!