Ashok Agarwal, Ph. D. , H. C. L. D. , Rakesh Sharma, Ph. D

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MiOXSYS: a novel method of measuring oxidation reduction potential in semen and seminal plasma Ashok Agarwal, Ph.D., H.C.L.D., Rakesh Sharma, Ph.D., Shubhadeep Roychoudhury, Ph.D., Stefan Du Plessis, Ph.D., Edmund Sabanegh, M.D. Fertility and Sterility Volume 106, Issue 3, Pages 566-573.e10 (September 2016) DOI: 10.1016/j.fertnstert.2016.05.013 Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Effect of sample type (semen or seminal plasma) and time (0 or 120 minutes) on sORP and cORP showing correlation of sORP in semen versus seminal plasma at (A) time 0 and (B) time 120 minutes; correlation of cORP in semen versus seminal plasma at (C) time 0 and (D) time 120 minutes; correlation of sORP at time 0 versus 120 minutes in (E) semen and (F) seminal plasma; correlation of cORP at time 0 versus time 120 minutes in (G) semen and (H) seminal plasma. Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 2 ROC curve establishing the cutoff in (A) semen and (B) seminal plasma. Distribution of sORP in subjects with normal and abnormal motility in (C) semen and (D) seminal plasma, suggesting a criterion for sORP in distinguishing semen quality based on good (≥40%) and poor (<40%) motility. Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 The MiOXSYS System. This device measures ORP using only a galvanostatic MiOXSYS analyzer (A) and disposable sensor strips (B). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Correlation of semen sORP with sperm parameters at time 0 in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 Correlation of seminal plasma sORP with sperm parameters at time 0 in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 4 Correlation of semen sORP with sperm parameters at time 120 in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 5 Correlation of seminal plasma sORP with sperm parameters at time 120 in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 6 Correlation of semen cORP with sperm parameters in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 7 Correlation of seminal plasma cORP with sperm parameters at time 0 in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 8 Correlation of semen cORP with sperm parameters at time 120 in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 9 Correlation of seminal plasma cORP with sperm parameters at time 120 in healthy controls (A, concentration; B, total sperm count; C, motility; D, morphology) and infertile patients (E, concentration; F, total sperm count; G, motility; H, morphology). Fertility and Sterility 2016 106, 566-573.e10DOI: (10.1016/j.fertnstert.2016.05.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions