FANCC Antibody Test Conclusions:

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Presentation transcript:

FANCC Antibody Test 1 2 3 4 1 2 3 4 Conclusions: Lane 1: FANCC-Flag 293 EBNA lys. Lane 2: 293 EBNA (-) Lane 3: FANCC-Flag 293 EBNA lys. Lane 4: 293 EBNA (-) 72  FANCC FANCC  55 Conclusions: C-2 is better than C-1, but both work C-2 may detect endogenous in 293 cells NOTE: C-1 has major cross reacting band near FANCC 1º ab FANCC-1 1º ab FANCC-2 1º ab Flag 2º ab anti-rabbit-HRP 2º ab anti-rabbit-HRP Alexis LaChapelle, 7-25-05 Hoatlin Lab

FANCD2 Antibody Test 1 2 3 4 1 2 3 4 Conclusions: Lane 1: FANCD2-Flag 293 EBNA lys. Lane 2: 293 EBNA (-) Lane 3: FANCD2-Flag 293 EBNA lys. Lane 4: 293 EBNA (-) FANCD2  170 Conclusions: Both antibodies work, and detect endogenous D2 Can’t resolve D2-S/D2-L in this experiment 1º ab FANCD2-1 (B9700) 1º ab FANCD2-2 (B9699) 1º ab FANCD2 (3686) 2º ab anti-rabbit-HRP 2º ab anti-rabbit-HRP Alexis LaChapelle, 7-25-05 Hoatlin Lab

FANCG Antibody Test 1 2 1 2 Conclusions: Lane 1: FANCG-HA 293 EBNA lys. Lane 2: 293 EBNA (-) Conclusions: G-1 no signal (not shown) G-2 very good and specific NOTE: Oddly, can’t detect endogenous FANCG in 293 cells. 100  FANCG 72  1º ab FANCG-2 1º ab HA 2º ab anti-rabbit-HRP Alexis LaChapelle, 7-25-05 Hoatlin Lab

FANCF Antibody Test 1 2 1 2 Conclusions: Lane 1: FANCF Precision Flag lys. Lane 2: 293 EBNA (-) FANCF 40  33  Conclusions: F very good and specific 1º ab FANCF 1º ab Flag 2º ab anti-rabbit-HRP Alexis LaChapelle, 7-25-05 Hoatlin Lab