Molecular Diagnosis of Clear Cell Sarcoma

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Presentation transcript:

Molecular Diagnosis of Clear Cell Sarcoma Cristina R. Antonescu, Sylvia J. Tschernyavsky, James M. Woodruff, Achim A. Jungbluth, Murray F. Brennan, Marc Ladanyi The Journal of Molecular Diagnostics Volume 4, Issue 1, Pages 44-52 (February 2002) DOI: 10.1016/S1525-1578(10)60679-4 Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Microscopic appearance of nested epithelioid cells with clear cytoplasm and prominent nucleoli, separated by fibrous bands and showing scattered multinucleated giant cells (hematoxylin & eosin; ×10). The Journal of Molecular Diagnostics 2002 4, 44-52DOI: (10.1016/S1525-1578(10)60679-4) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Case CCS 6 showed a distinct microscopic appearance, with epithelioid cells, showing a higher degree of nuclear pleomorphism and anaplasia, arranged in an alveolar pattern of growth, due to a striking loss of cohesion (hematoxylin & eosin; ×20). The Journal of Molecular Diagnostics 2002 4, 44-52DOI: (10.1016/S1525-1578(10)60679-4) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Immunohistochemical study with D5 monoclonal antibody showing strong and diffuse nuclear labeling in the tumor cells and also in the multinucleated giant cells (×20). The Journal of Molecular Diagnostics 2002 4, 44-52DOI: (10.1016/S1525-1578(10)60679-4) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Ultrastructural appearance of case CCS 4 with few stage II melanosomes (arrows) and stage III pigmented melanosomes (arrowheads) (×29,400). The Journal of Molecular Diagnostics 2002 4, 44-52DOI: (10.1016/S1525-1578(10)60679-4) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 5 Detection of EWS-ATF1 transcripts in CCS by RT-PCR using RNA extracted from frozen tissues. M1: Size marker (HaeIII digest of ΦX174);M2: 100-bp DNA ladder; ±, reverse transcriptase added/not added (the latter representing the “no RT” negative control). The Journal of Molecular Diagnostics 2002 4, 44-52DOI: (10.1016/S1525-1578(10)60679-4) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 6 Detection of EWS-ATF1 transcripts in CCS by nested RT-PCR using RNA extracted from paraffin-embedded tissues. Top panel shows products of RT-PCR (first step); bottom panel shows products of nested PCR performed on products of first step. M:HaeIII digest of ΦX174. CCS + ctrl: RNA extracted from frozen tissue in CCS 1, used as positive control. CCS 11, a and b: different paraffin blocks used for RNA extraction. ±, reverse transcriptase added/not added (the latter representing the “no RT” negative control). The Journal of Molecular Diagnostics 2002 4, 44-52DOI: (10.1016/S1525-1578(10)60679-4) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 7 Detection of melanocyte-specific (M) and consensus (C) MITFtranscripts in CCS by RT-PCR. RNA from the SK-MEL-19 melanoma cell line provided a positive control. M1: Size marker (HaeIII digest of ΦX174);M2: 100-bp DNA ladder; ±, reverse transcriptase added/not added (the latter representing the “no RT” negative control). The Journal of Molecular Diagnostics 2002 4, 44-52DOI: (10.1016/S1525-1578(10)60679-4) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions