PLASMID ISOLATION AND ANALYSIS Part I Overnight Culture Suspension

Overnight Culture Suspension Step 1 Label a sterile 50 ml Conical tube with your name and date. PLASMID ISOLATION AND ANALYSIS: Part I

Overnight Culture Suspension Step 2 Do a sterile transfer of one sterile pipette full of LB broth containing ampicillin antibiotic into the Conical tube. PLASMID ISOLATION AND ANALYSIS: Part I

Overnight Culture Suspension Step 3 Locate a well-formed colony on your plate of transformed bacteria. Use a sterile inoculating loop, bent paper clip, or toothpick to scrape up the colony and transfer it to the LB broth in the tube. PLASMID ISOLATION AND ANALYSIS: Part I

Overnight Culture Suspension Step 4 Incubate the tube for 24 hours in a 37oC shaking water bath or 48 hours in a 37oC incubator with occasional shaking by hand (shake the tube a couple of times an hour when possible). PLASMID ISOLATION AND ANALYSIS: Part I

Overnight Culture Suspension Step 5 After the overnight growth period is complete, proceed to part II, Plasmid Purification. PLASMID ISOLATION AND ANALYSIS: Part I