Lab Exercise 7: Acid Fast Staining.

Slides:

Advertisements

Similar presentations

Ex. 5: Acid-Fast and Endospore Stains

Advertisements

BACTERIAL STAINING.

1 Module AFB MICROSCOPY. 2 Content Overview Collection of sputum specimens Quality of sputum specimens Processing of sputum specimens Registration of.

Faculty of Medicine and Health Sciences Microbiology Lab second semester 2013 prepared by: Mohammad Al-Qadi

Observation of bacteria using staining procedures Simple staining Gram staining.

Differential Stains (Gram stain & Acid Fast Stain) Abdelraheem BA

Gram Staining Method Biology 2 Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Inoculating Loop or Wood Splint Paper Towels.

Third lecture for microbiology manual

Bacterial Staining General Discussion. Stains All dyes are salts –Ionize Cationic Anionic Techniques –Single dyes –Multiple dyes Use.

Negative Stain and Acid Fast Stain

S TAINING M ICROORGANISMS An overview of staining.

Lab 4 Goals and Objectives: ***Use wax pencils (China Marker): Sharpie will wash off with alcohol! Be sure to resuspend broth cultures completely! Exercise.

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

PHT 226 Lab # 3 Gram’s stain (mixture) Acid fast stain Spore stain.

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

Lab #5. Review for Practical #1 Colony morphology (pg. 19)

Microbiology Professor Sidelsky 2007

Gram Stain Differential stain (Hans Christian Gram, a Danish doctor ). He developed a new method to stain bacteria so they can be visible in specimen.

Lab #4. Review of Lab #3 Oxygen requirements Obligate aerobes (B. subtilis) Obligate anaerobes (C. sporogenes) Facultative anaerobes (E. coli, K. pneumoniae,

Gram’s stain (mixture)

StainsStains. Cheek Cells Without Stain Cheek Cells with Simple Stain (Methylene Blue)

Lab#10 Basmah Almaarik.  It has many species one of them is Mycobacterium tuberculosis that cause TB.  Some other common species seen include:  M.

Staining Lab 3 and 4 Notes and Pictures. Smears Air dry first to prevent lysis (boiling) Heat Fixing –Kill –Stops autolysis –Adherence to slide.

Lab 4 Lab 3 Goals and Objectives

PREPARATION OF A SMEAR GRAM STAIN ACID FAST STAIN

Practical seminar 4 Acid fast staining - Mycobacterium tuberculosis

Lab # 3 Gram and Acid Fast stain Medgar Evers College Biology 261 Prof. Santos.

Differential staining

 Prepare a separate smears of E. coli and S. aureus on two different slides. Fix these preparation by allowing them to dry by passing over the Bunsen.

Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the acid fast.

Gram staining Techniques. Some history Bacteria are translucent Bacteria are translucent Staining make them visible under microscope Staining make them.

TOPICS Staining and stain Staining and stain Types of staining techniques Types of staining techniques Gram Staining - Principles Gram Staining - Principles.

StainsStains. Negative Stain Cheek Cells Without Stain.

Observing Microorganisms through a Microscope

Observing Microorganisms through a Microscope Dr. Bhavesh Patel Principal V.P. and R.P.T.P. Science College Vallabh Vidyanagar –

Gram Staining Method Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Innoculating Loop Paper Towels.

Differential staining

Neisseria + AFB.

GRAM and ACID FAST STAINS

PRACTICAL GRAM STAINING

Principles of Laboratory Diagnosis of Infectious Diseases

Gram Staining: Principle, Procedure and Results MSc. Sarah Ahmed

Gram’s stain Acid fast stain Spore stain Hanging drop technique

Biology and Biotechnology department

PRACTICAL GRAM STAINING

THE GRAM STAIN.

Chapter 40 Staining Specimens

2 loops each organism Wax Pencil mixed together

PRACTICAL GRAM STAINING

Practical 2 Gram staining.

Week 4W Review cultures: Exp 2B Lab Two & Exp 3 Lab Two

Cocci. Cocci Bacilli Spirochetes Staphylococcus Arrangement.

MICROSCOPIC EXAMINATION OF BACTERIA (UNSTAINED & STAINED SMEARS)

تشخیص آزمایشگاهی بیماری سل

Special Staining Special stains allow us to make special structures visible that might not otherwise be visible using other staining techniques. 4 Special.

Biology and Biotechnology department

Observing Microorganisms through a Microscope

Observing Microorganisms Through a Microscope

Lab Exercise 7: Acid Fast Staining.

Practical No. 5 Staining Department of Microbiology

Simple Staining and Bacterial Cell Morphology

Observing Microorganisms Through a Microscope

Acid Fast Staining.

Types of microbiologic stains

Presentation transcript:

Lab Exercise 7: Acid Fast Staining

Kinyoun Acid-Fast Staining Procedure Carbol fuchsin Acid alcohol Methylene blue Reddish-pink Blue Acid Fast Nonacid Fast Kinyoun Acid-Fast Staining Procedure 1. A sample of cells is mixed with a drop of water on a clean slide to make a smear. After air drying, the slide is heat fixed. 2. Slide is flooded with carbol fuchsin (primary stain basic fuchsin + mordant carbolic acid) and allowed to sit for 15 minutes. Slide is rinsed until water coming off the slide is clear. 3. Slide is decolorized with acid alcohol (3% HCl and 95% alcohol) 20 seconds, then rinsed. 4. Slide is flooded with methylene blue (counter stain) for 60 seconds and then rinsed.

Note the reddish-pink color and “cording” (sticking together in long ropy masses) of the Mycobacterium cells due to the excess lipids of the cell wall

Identify the following in this sputum smear: Epithelial cell (E), Streptococcus mutans (S), Mycobacterium tuberculosis (M) M S E

Locate Mycobacterium tuberculosis (M)

Put these solutions in order of their use in the Kinyoun acid-fast staining procedure B C Answer: C, B, A