Pre-Breeding and Trait Discovery Flagship Program 5 Pre-Breeding and Trait Discovery Rajeev Gupta, PhD (Cantab) Principal Scientist & Theme Leader-Genomics & Trait Discovery, ICRISAT FP5 Leader- CGIAR Research Program on Grain Legumes & Dryland Cereals (GLDC)

FP5: Vision & Mission “To develop and deploy modern genetic, omics (genomic, phenomic, etc.) molecular resources and tools for structural, comparative, translational and functional genomics approaches for mapping, dissection and characterization of target traits leading to marker/allele/events discovery and improved processes for deployment in breeding to accelerate the rate of genetic gain in GLDC crops”

FP5: Core Team An excellent team with many members! Flagship Program 5 (FP5): Pre-breeding & Trait Discovery Leader Rajeev Gupta (ICRISAT, India) CoA 5.1 Pre-breeding Ousmane Boukar (IITA, Kano) Jean-Francois Rami (CIRAD) CoA 5.2 Trait discovery Damaris Odeny (ICRISAT, Nairobi) Laurent Laplaze (IRD) CoA 5.3 Enabling technologies Pooja Bhatnagar (ICRISAT) Aladdin Hamwieh (ICARDA) An excellent team with many members! Going forward: CoA specific sub teams

FP5: Cluster of Activities (CoAs) Major activity CoA 5.1: Pre-breeding Use of natural (e. g, in un-adapted germplasm) or induced (e. g., mutants populations) diversity available in key priority traits in GLDC crops CoA: 5.2 Trait discovery Trait discovery, dissection and elucidation with molecular, omics, biochemical and physiological tools to provide markers/alleles/events for use in breeding CoA 5.3: Enabling technologies Modern enabling tools, technologies, and platforms to facilitate efficient trait discovery and breeding

CoA 5.1: Pre-Breeding Examples of Traits

CoA5.2: Trait Discovery Prioritized Target Traits in GLDC Crops

CoA 5.3: Enabling Tools and Technologies

Stages of Discovery & Deployment in Crop Improvement Pipeline STAGE A IDEA CONCEPTION STAGE B PROOF OF CONCEPT STAGE C OPTIMIZATION STAGE D DEPLOYMENT INFORMED DECISIONS FP5 FP4 ‘Fail Fast’: Drop non-promising projects/activities ‘sooner the better’

FP5: Impact Pathway

Thank you Demand-driven Innovation for the Drylands www.gldc.cgiar.org In partnership with CGIAR Centers, public and private organizations, governments, and farmers worldwide www.gldc.cgiar.org Thank you

Major Current Constraints to Enhance Genetic Gains in GLDC crops Limited genetic variability in the germplasm for some important traits (e. g., Helicoverpa resistance in chickpea and pigeonpea, dry root rot resistance in chickpea) Barriers to interspecific hybridizations in chickpea, groundnut, pigeonpea etc. Lack of varieties with consumer preferred traits such as grain nutritional value, cooking quality, reduced post harvest losses for market demand Unavailability of high-throughput and low cost genotyping platform in most GLDC crops Unavailability of high-throughput diagnostic markers for key traits in most of GLDC crops Lack of high throughput & cutting edge genetic engineering platforms such as genome editing High throughput precision phenotyping platforms in most of the key traits for GLDC crops

Intermediate Indicators of Success for Various Groups of Traits Group of Traits Intermediate indicators of success Abiotic stress tolerance Drought QTLs fine mapped to identify gene responsible, HTP markers available and deployed in forward breeding, Biotic stresses tolerance Transgenic events for insect resistance available in chickpea and pigeonpea. Validated HTP markers available for key abiotic stress tolerance traits in all crops. Improved grain nutritional quality HTP markers developed for protein content in chickpea and pigeonpea, oil content in groundnut and Fe and Zn content in at least four GLDC crops. Post-harvest losses/consumer-preferred traits  Sources of resistance identified for rancidity in pear millet and aflatoxin contamination in groundnut.

FP5: Break Out Session W1/W2 is very-very limited and 80% to used to cover salaries Identify the priority activities/projects in each CoA for 2018 & beyond Leverage funds available in current bilateral and estimate gap funding Check with partners for partnership to deliver Priority list of activities for 2018 Priority list for long term activities RMC makes final call of funding support Innovation fund $0.5M for 2018 Partners can leverage More details to come from RMC

FP5: Break Out Session CoA breif description of activity/project 2018 or 2018+ Bilateral funding $ Gap funding $ lead & Interested/potential partners (committed Y/N) Expected outcome (with time line)