Robert J. Pitsch, MD, David J. Minion, MD, Margaret L

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Presentation transcript:

Platelet-derived growth factor production by cells from Dacron grafts implanted in a canine model Robert J. Pitsch, MD, David J. Minion, MD, Margaret L. Goman, BS, John A. van Aalst, MD, Paul L. Fox, PhD, Linda M. Graham, MD Journal of Vascular Surgery Volume 26, Issue 1, Pages 70-78 (July 1997) DOI: 10.1016/S0741-5214(97)70149-6 Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Southern blots of SMC and EC PDGF A-chain and GAPDH mRNA amplified by RT-PCR. Aortic and graft ECs and SMCs are from a representative dog. Images are computer-enhanced. A-chain/GAPDH numbers represent the ratios of signal intensities before enhancement. Journal of Vascular Surgery 1997 26, 70-78DOI: (10.1016/S0741-5214(97)70149-6) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Graph of RT-PCR results shows PDGF A-chain/GAPDH signal intensity. Graft ECs express lower levels of PDGF A-chain mRNA than aortic ECs, whereas graft SMCs consistently express higher levels than aortic SMCs. Journal of Vascular Surgery 1997 26, 70-78DOI: (10.1016/S0741-5214(97)70149-6) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Immunohistochemical analysis using antibody to PDGF A-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery 1997 26, 70-78DOI: (10.1016/S0741-5214(97)70149-6) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Immunohistochemical analysis using antibody to PDGF A-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery 1997 26, 70-78DOI: (10.1016/S0741-5214(97)70149-6) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Immunohistochemical analysis using antibody to PDGF B-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery 1997 26, 70-78DOI: (10.1016/S0741-5214(97)70149-6) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Immunohistochemical analysis using antibody to PDGF B-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery 1997 26, 70-78DOI: (10.1016/S0741-5214(97)70149-6) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions