Figure 1 Metal bioimaging in 30-μm thick cryosections taken from the brains of untreated and AAV8-AAT-co-miATP7B-treated Atp7b null mouse Metal bioimaging.

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Figure 1 Metal bioimaging in 30-μm thick cryosections taken from the brains of untreated and AAV8-AAT-co-miATP7B-treated Atp7b null mouse Metal bioimaging in 30-μm thick cryosections taken from the brains of untreated and AAV8-AAT-co-miATP7B-treated Atp7b null mouse The contents of copper (Cu), iron (Fe), zinc (Zn), manganese (Mn), sodium (Na), magnesium (Mg), potassium (K), calcium (Ca), phosphorus (P), sulfur (S), carbon (C), chromium (Cr), nickel (Ni), and lead (Pb) in each section are shown. Individual images of elements were done with the ELAI software tool.7 Light microscopic (LM) images of cryosections and pictures of brains analyzed are shown for orientation in the left margin. In this analysis, Atp7b+/− mice served as a further control. Please note that the content of C serving as reference is given in %, whereas concentrations of all other elements are given in μg/g liver tissue. Details about animal manipulation are given elsewhere.2 Ricarda Uerlings et al. Neurol Genet 2018;4:e243 Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.