CRISPR-Cas9 gene drive system

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Presentation transcript:

CRISPR-Cas9 gene drive system

What is a gene drive? Elements that increase the odd that they will be inheritance Mendelian inheritance Gene drive inheritance Natural gene drives Synthetic gene drives -Transposable elements -Maternal effect killers -Homing endonucleases Limitations: Engineering nucleases to cut new targets

CRISPR/CAS9 system Jennifer Doudna Guide RNA Spacer PAM sequence Protospacer Cas9 Genomic DNA

CRISPR/CAS9 gene drive system Kevin Esvelt A D B E C F

Inheritance Esvelt et al. 2014

Limitations 1. Asexual organisms can`t be affected 2. Many generations to spread through populations Ineffective in long lived organisms 3. Drive-mediated genome alterations aren`t permanent

Capabilities 1. Reversibility 2. Population suppression Esvelt et al. 2014 2. Population suppression Deleterious changes Bias the population towards one sex 3. Complex alterations Sensitize Speciate

Applications Prevent mosquitoes from transmitting malaria AgCPA Valentino Gantz AgCPA

THANKS FOR YOUR ATTENTION!