The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

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Presentation transcript:

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large Cell Lymphoma and Other Peripheral T-Cell Lymphomas Brent T. Tan, Katie Seo, Roger A. Warnke, Daniel A. Arber The Journal of Molecular Diagnostics Volume 10, Issue 6, Pages 502-512 (November 2008) DOI: 10.2353/jmoldx.2008.080054 Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Histology of representative ALCL and pre-B-ALL cases. All panels shown at identical magnification. a–e: ALK+ ALCL stained with H&E (a), immunohistochemistry with anti-CD2 (b), anti-CD30 (c), in situ hybridization for EBV (d), or anti-ALK (e). f–i: ALK− ALCL stained with H&E (f), anti-CD3 (g), in situ hybridization for EBV (h), or anti-CD30 (i). j–l: pre-B-ALL stained with H&E (j), anti-CD79a (k), or anti-TdT (l). Images were captured with a Nikon Eclipse E1000 microscope (Nikon, Tokyo, Japan) using a 60×/0.95 objective lens (Nikon) and a Spot CCD camera (Diagnostic Instruments, McHenry, IL) using Spot software version 4.6 for image acquisition. The Journal of Molecular Diagnostics 2008 10, 502-512DOI: (10.2353/jmoldx.2008.080054) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Electropherograms of PCRs for IGH rearrangements. The x axis indicates DNA length in nucleotides with expected product sizes above the axis. The y axis indicates relative fluorescence units. Shown are amplification products from a case of ALK− ALCL. a, b, and c represent different multiplex PCRs that use primers that bind to the FR1 (a), FR2 (b), or FR3 (c) region of IGH. This case was interpreted as positive with clonal peaks (arrows) detected by FR1, FR2, and FR3 primers. The Journal of Molecular Diagnostics 2008 10, 502-512DOI: (10.2353/jmoldx.2008.080054) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Electropherograms of PCRs for TRG rearrangements. The x axis indicates DNA length in nucleotides with expected product sizes above the axis. The y axis indicates relative fluorescence units. a: A case of pre-B-ALL with a clonal peak (arrow) detected in the multiplex PCR that uses primers to detect rearrangements involving Vγ10 or Vγ1-8 of TRG. b: An ALK− ALCL case with a clonal peak (arrow) detected in the multiplex PCR that uses primers to detect rearrangements involving Vγ11 or Vγ9 of TRG. The Journal of Molecular Diagnostics 2008 10, 502-512DOI: (10.2353/jmoldx.2008.080054) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions