Monocyte chemoattractant chemokines in cystic fibrosis

Slides:

Advertisements

Similar presentations

Volume 94, Issue 2, Pages (March 2014)

Advertisements

CXCR6 and CCR5 Localize T Lymphocyte Subsets in Nasopharyngeal Carcinoma Greg Parsonage, Lee Richard Machado, Jan Wai-Ying Hui, Andrew McLarnon, Tilo.

Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.

Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor Preston E. Bratcher, Steven M. Rowe, Ginger.

Volume 61, Issue 2, Pages (February 2012)

Reduced Frequency of Regulatory T Cells in Peripheral Blood Stem Cell Compared to Bone Marrow Transplantations Céline Blache, Joe-Marc Chauvin, Aude.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy Yin Yao, MD, Cai-Ling.

Two cases of disseminated Mycobacterium avium infection associated with a new immunodeficiency syndrome related to CXCR4 dysfunctions A.-V. Doncker,

Reduced endothelial progenitor cells in extracranial arterial stenosis but not intracranial arterial stenosis Zhixin Huang, MD, PhD, Xiaohao Zhang, MD,

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection

Reduced levels of active GLP-1 in patients with cystic fibrosis with and without diabetes mellitus Magnus Hillman, Leif Eriksson, Lena Mared, Karin Helgesson,

Neil E. Alexis, Marianne S. Muhlebach, David B. Peden, Terry L. Noah

Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus.

Cord Blood–Derived and Peripheral Blood–Derived Cytokine-Induced Killer Cells Are Sensitive to Fas-Mediated Apoptosis Ludovic Durrieu, Mame Massar Dieng,

Is infection with hypermutable Pseudomonas aeruginosa clinically significant? Adi Auerbach, Eitan Kerem, Marc Victor Assous, Elie Picard, Maskit Bar-Meir

Zachary M. Sellers, Lori McGlocklin, Andrea Brasch

William G. Flight, Jonathan Shaw, Susan Johnson, A

Type 2 innate lymphoid cells in induced sputum from children with severe asthma Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis Rahul Purwar, Thomas Werfel, Miriam.

Receptor Activator of NF-κB Ligand Promotes the Production of CCL17 from RANK+ M2 Macrophages Taku Fujimura, Yumi Kambayashi, Sadanori Furudate, Masayuki.

H. Grasemann, E. Tullis, F. Ratjen Journal of Cystic Fibrosis

Single high-dose oral vitamin D3 (stoss) therapy — A solution to vitamin D deficiency in children with cystic fibrosis? Darren Shepherd, Yvonne Belessis,

Pseudomonas aeruginosa in cystic fibrosis: Pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease Malin Carlsson, Swati.

Effect of First-Line Treatment on Myeloid-Derived Suppressor Cells’ Subpopulations in the Peripheral Blood of Patients with Non–Small Cell Lung Cancer

Measures of body habitus are associated with lung function in adults with cystic fibrosis: A population-based study Doug L. Forrester, Alan J. Knox,

Raphaël Chiron, Y. Yaël Grumbach, Nga V. T

Statistical limitations of percent ideal body weight as measure for nutritional failure in patients with cystic fibrosis T.O. Hirche, H. Hirche, S. Jungblut,

Cirrhosis and other liver disease in cystic fibrosis

Katelyn Krivchenia, Don Hayes, Joseph D. Tobias, Dmitry Tumin

Increased serum concentration of G-CSF in cystic fibrosis patients with chronic Pseudomonas aeruginosa pneumonia P.Ø. Jensen, C. Moser, A. Kharazmi,

Jerome A. Sigua, MD, Becky Buelow, MD, Dorothy S

Amanda L. Brennan, Khin M. Gyi, David M

Safety and early treatment effects of the CXCR2 antagonist SB in patients with cystic fibrosis Richard B. Moss, Sunil J. Mistry, Michael W. Konstan,

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

Fiona K. Dunlevy, S. Lorraine Martin, Francine de Courcey, J

D. Stevens, P.J. Oades, N. Armstrong, C.A. Williams

Claire J. Tipping, Rebecca L. Scholes, Narelle S. Cox

Vitamin A and lung function in CF

Psoriasis Is Characterized by Accumulation of Immunostimulatory and Th1/Th17 Cell- Polarizing Myeloid Dendritic Cells Lisa C. Zaba, Judilyn Fuentes-Duculan,

Increased, but Functionally Impaired, CD14+ HLA-DR–/low Myeloid-Derived Suppressor Cells in Psoriasis: A Mechanism of Dysregulated T Cells David C. Soler,

A.H. Gifford Journal of Cystic Fibrosis

Human Plasmacytoid Dendritic Cells Express Receptors for Anaphylatoxins C3a and C5a and Are Chemoattracted to C3a and C5a Ralf Gutzmer, Brigitta Köther,

Tregony Simoneau, Gregory S. Sawicki, Carly E. Milliren, Henry A

M. Al-Aloul, M. Jackson, G. Bell, M. Ledson, M. Walshaw

Small macrophages are present in early childhood respiratory disease

E. Tschiedel, H. Grasemann, F. Ratjen Journal of Cystic Fibrosis

Postoperative changes in blood monocyte subsets.

A Novel Soluble Form of Tim-3 Associated with Severe Graft-versus-Host Disease John A. Hansen, Samir M. Hanash, Laura Tabellini, Chris Baik, Richard L.

Pharmacokinetics and sputum penetration of azithromycin during once weekly dosing in cystic fibrosis patients E.B. Wilms, D.J. Touw, H.G.M. Heijerman

Theresa A. Laguna, Cynthia B. Williams, Kyle R

Nutritional decline in cystic fibrosis related diabetes: The effect of intensive nutritional intervention H. White, K. Pollard, C. Etherington, I. Clifton,

A new method of sweat testing: the CF Quantum®sweat test

Human Beta-Defensin 3 Induces Maturation of Human Langerhans Cell–Like Dendritic Cells: An Antimicrobial Peptide that Functions as an Endogenous Adjuvant

J.E. Spahr, R.B. Love, M. Francois, K. Radford, K.C. Meyer

Daniel J. Smith, Gregory J. Anderson, Scott C. Bell, David W. Reid

(A) HLA-E expression on the surface of CD14+ cells in patients with AS, HLA-B*2705, HLA-B*2709-positive and HLA-B27-negative HCs. Results are expressed.

Allergen-specific CD8+ T cells in peanut-allergic individuals

Decreased interleukin-18 expression in BAL cells and peripheral blood mononuclear cells in adult cystic fibrosis patients Hans-Peter Hauber, Inga S Beyer,

D.F. Waterhouse, A.M. McLaughlin, C.G. Gallagher

Figure 1 CD52 expression on innate myeloid and lymphoid cell subsets

Experience using centralized spirometry in the phase 2 randomized, placebo-controlled, double-blind trial of denufosol in patients with mild to moderate.

CFTR modulators and pregnancy: Our work has only just begun

Airway inflammation in mild cystic fibrosis

N Dauletbaev, K Viel, R Buhl, T.O.F Wagner, J Bargon

Volume 84, Issue 1, Pages (July 2013)

Expression of fractalkine and its receptor, CX3CR1, in atopic dermatitis: Possible contribution to skin inflammation Takeshi Echigo, MD, Minoru Hasegawa,

Expression of Sézary Biomarkers in the Blood of Patients with Erythrodermic Mycosis Fungoides Charlotte Hurabielle, Laurence Michel, Caroline Ram-Wolff,

R. D. Gray, M. Imrie, A. C. Boyd, D. Porteous, J. A. Innes, A. P

Abnormal monocyte distribution and loss of HLA-DR in patients with stage IV melanoma. Abnormal monocyte distribution and loss of HLA-DR in patients with.

Presentation transcript:

Monocyte chemoattractant chemokines in cystic fibrosis Satish Rao, Adam K.A. Wright, William Montiero, Loems Ziegler-Heitbrock, Jonathan Grigg Journal of Cystic Fibrosis Volume 8, Issue 2, Pages 97-103 (March 2009) DOI: 10.1016/j.jcf.2008.09.009 Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

Fig. 1 Determination of absolute numbers of blood monocytes by flow cytometry. Peripheral blood leucocytes are identified using flow cytometry by light scatter characteristics. Mononuclear cells are identified in gate R2 (A). Gate R3 (A) is placed around flow-count beads. Events in R2 are gated onto a CD14/HLA DR dot plot. Gate R4 (B) defines events that express HLA DR and CD14. Events in R4 are then given in the CD14 vs. CD16 dot plot (C). CD14++CD16− monocytes are represented in R6 and CD14+CD16+ monocytes in gate R5. The absolute count is derived from the number of flow-count beads represented in gate R8. Journal of Cystic Fibrosis 2009 8, 97-103DOI: (10.1016/j.jcf.2008.09.009) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

Fig. 2 A) Representative histograms of CCR2 and CX3CR1 expression on CD14++CD16− blood monocytes from CF and control subjects. The shaded histogram and open histograms denote non-specific background staining and specific antibody staining respectively. Median fluorescence intensity is calculated from the difference between specific staining (open histogram) and non-specific staining (shaded). Both CCR2 and CX3CR1 are expressed on both CF and control CD14++CD16− monocytes but there was no difference between the groups. B) Representative histograms of CCR2 and CX3CR1 expression on CD14+CD16+ blood monocytes from CF and control subjects. The shaded histogram and open histograms denote non-specific background staining and specific antibody staining respectively. Median fluorescence intensity is calculated from the difference between specific staining (open histogram) and non-specific staining (shaded). CX3CR1 but not CCR2 is expressed on both CF and control CD14+CD16+ monocytes. There was no difference between the groups in CX3CR1 expression. Journal of Cystic Fibrosis 2009 8, 97-103DOI: (10.1016/j.jcf.2008.09.009) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

Fig. 3 A) Dot plot showing concentrations of blood CCL2, formally called monocyte chemoattractant protein-1, in cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. ⁎p<0.01 by Mann Whitney test. B) Concentrations of CX3CL1, formally called fractalkine, in blood from cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. There is no significant difference between the groups by Mann Whitney Test. Journal of Cystic Fibrosis 2009 8, 97-103DOI: (10.1016/j.jcf.2008.09.009) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

Fig. 4 A) Dot Plot showing concentrations of CCL2 in induced sputum of cystic fibrosis patients compared with healthy controls. The horizontal bar represents the median value. ⁎p<0.05 by Mann Whitney test. B) Concentrations of CX3CL1 in sputum of cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. There is no significant difference between the groups by Mann Whitney test. Journal of Cystic Fibrosis 2009 8, 97-103DOI: (10.1016/j.jcf.2008.09.009) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions