Fatty Acid Oxidation Title slide - fatty acid oxidation, beta oxidation

The Knoop experiment that proved beta oxidation

Fatty acid must be activated in an ATP-dependent reaction Fatty acid must be activated in an ATP-dependent reaction. An acyladenylate is formed as an intermediate - anhydride between the fatty acid and AMP. This is a two step reaction; the activated acyl group from the acyl adenylate is transferred to CoA.

Carnithine is required for the transport of the acyl group into the mitochondria where beta oxidation occurs.

The carrier is located in the membrane and vectorially transports the acyl group.

The reaction sequence in fatty acid oxidation - one cycle; this is repeated and two carbons are removed at a time.

The first reaction is catalyzed by acyl~CoA dehydrogenase, a flavin-depedent enzyme.

Further steps

In the second reaction the dienoylCoA is reduced using NADPH.

Thiolase transfers to a C-two unit to CoA

Ketone body - acetoacetate is reduced to beta-hydroxdybutyrate.

Hypoglcin inhibits the acyl~CoA dehydrogenase that ties up the FAD.

Reaction mechanism

How to handle complications