Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation by Pieternella S. in `t Anker, Sicco A. Scherjon, Carin Kleijburg-van der Keur, Willy A. Noort, Frans H. J. Claas, Roelof Willemze, Willem E. Fibbe, and Humphrey H. H. Kanhai Blood Volume 102(4):1548-1549 August 15, 2003 ©2003 by American Society of Hematology
The Dynal Reli SSO (Dynal Biotech, Hamburg, Germany) reverse line blot strip assay was used for molecular typing of the HLA-A and HLA-B locus alleles of maternal cells, fetal cells, and culture-expanded amniotic fluid–derived MSCs from the same sample. The Dynal Reli SSO (Dynal Biotech, Hamburg, Germany) reverse line blot strip assay was used for molecular typing of the HLA-A and HLA-B locus alleles of maternal cells, fetal cells, and culture-expanded amniotic fluid–derived MSCs from the same sample. The HLA-A and HLA-B type of the culture-expanded amniotic fluid–derived MSCs is identical to the fetal HLA-A and HLA-B type and mismatched with the maternal HLA-A and HLA-B type. Upward arrows indicate maternal specific HLA antigens, and downward arrows indicate fetal-specific HLA antigens. Pieternella S. in `t Anker et al. Blood 2003;102:1548-1549 ©2003 by American Society of Hematology