Induction of mesenchymal/epithelial marker expression in human amniotic fluid stem cells Nicol Siegel, Alessandro Valli, Christiane Fuchs, Margit Rosner,

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Induction of mesenchymal/epithelial marker expression in human amniotic fluid stem cells Nicol Siegel, Alessandro Valli, Christiane Fuchs, Margit Rosner, Markus Hengstschläger Reproductive BioMedicine Online Volume 19, Issue 6, Pages 838-846 (December 2009) DOI: 10.1016/j.rbmo.2009.09.015 Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Schematic presentation of the experimental procedure. Human amniotic fluid cells were cultivated in F-10 nutrient mixture medium, followed by cultivation in mesenchymal differentiation medium containing epidermal growth factor and platelet-derived growth factor BB and by cultivation in epithelial differentiation medium containing fibroblast growth factor 4 and hepatocyte growth factor. Reproductive BioMedicine Online 2009 19, 838-846DOI: (10.1016/j.rbmo.2009.09.015) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Reverse-transcription polymerase chain reaction gene expression analyses in human amniotic fluid cells, cultivated in nutrient mixture medium (HAFC), cultivated in mesenchymal differentiation medium (mesench. diff.) and in epithelial differentiation medium (epithel. diff.). Two sets of representative amniotic fluid cell examples are shown for each gene. For the negative controls, water was used instead of cDNA. For each gene, cells were characterized for use as positive controls due to a significantly detectable specific mRNA expression (cells used are indicated in parentheses). Reproductive BioMedicine Online 2009 19, 838-846DOI: (10.1016/j.rbmo.2009.09.015) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Reverse-transcription polymerase chain reaction analysis of CD44 gene expression in different amniotic fluid cell samples cultivated sequentially in nutrient mixture medium, mesenchymal differentiation medium and epithelial differentiation medium. Reproductive BioMedicine Online 2009 19, 838-846DOI: (10.1016/j.rbmo.2009.09.015) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions