New strategy for the prenatal detection/exclusion of paternal cystic fibrosis mutations in maternal plasma  Ana Bustamante-Aragones, Jesus Gallego-Merlo, Maria Jose Trujillo-Tiebas, Marta Rodriguez de Alba, Cristina Gonzalez-Gonzalez, Guillermo Glover, Dan Diego-Alvarez, Carmen Ayuso, Carmen Ramos  Journal of Cystic Fibrosis  Volume 7, Issue 6, Pages 505-510 (November 2008) DOI: 10.1016/j.jcf.2008.05.006 Copyright © 2008 European Cystic Fibrosis Society Terms and Conditions

Fig. 1 Electropherograms of the parental DNA controls (Mother, as negative control and Father, as positive control) and the maternal plasma samples (Plasma) of each case. x axis, computed length of PCR products in base pairs as determined automatically by the use of internal lane standard; y axis, fluorescence intensities in arbitrary units. The arrows show the peak which represents the mutation. Case 1: Wild-type and mutated nucleotide (paternal mutation) are present in the plasma sample. Case 2: The electropherogram of the plasma sample is similar to the one presented by the maternal DNA, only the wild-type nucleotide is present. Case 3: In Plasma 1 (sample collected during the 12th week of gestation) and in Plasma 2 (sample collected during the 15th week of gestation), the peak associated with the paternal mutation is detected. Journal of Cystic Fibrosis 2008 7, 505-510DOI: (10.1016/j.jcf.2008.05.006) Copyright © 2008 European Cystic Fibrosis Society Terms and Conditions