Intra-articular injection of the cyclooxygenase-2 inhibitor parecoxib attenuates osteoarthritis progression in anterior cruciate ligament-transected knee.

Slides:

Advertisements

Similar presentations

Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model M. Saito, T.

Advertisements

B. Bai, Y. Li Osteoarthritis and Cartilage

Early genetic restoration of lubricin expression in transgenic mice mitigates chondrocyte peroxynitrite release and caspase-3 activation K.M. Larson,

Optical imaging of mouse articular cartilage using the glycosaminoglycans binding property of fluorescent-labeled octaarginine K. Inagawa, T. Oohashi,

The impact of early intra-articular administration of interleukin-1 receptor antagonist on lubricin metabolism and cartilage degeneration in an anterior.

Loss of extracellular matrix from articular cartilage is mediated by the synovium and ligament after anterior cruciate ligament injury C.M. Haslauer,

Adiabatic rotating frame relaxation of MRI reveals early cartilage degeneration in a rabbit model of anterior cruciate ligament transection J. Rautiainen,

Alleviation of osteoarthritis by calycosin-7-O-β-d-glucopyranoside (CG) isolated from Astragali radix (AR) in rabbit osteoarthritis (OA) model S.I. Choi,

Micromechanical mapping of early osteoarthritic changes in the pericellular matrix of human articular cartilage R.E. Wilusz, S. Zauscher, F. Guilak

Osteoporosis increases the severity of cartilage damage in an experimental model of osteoarthritis in rabbits E. Calvo, M.D., S. Castañeda, M.D., R.

Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis T. Weng, Y. Xie, L. Yi, J. Huang,

Maturation-dependent change and regional variations in acoustic stiffness of rabbit articular cartilage: an examination of the superficial collagen-rich.

Histopathological subgroups in knee osteoarthritis

Glucosamine sulfate reduces experimental osteoarthritis and nociception in rats: association with changes of mitogen-activated protein kinase in chondrocytes

Evaluation of histological scoring systems for tissue-engineered, repaired and osteoarthritic cartilage M. Rutgers, M.J.P. van Pelt, W.J.A. Dhert, L.B.

Long-term effect of sodium hyaluronate (Hyalgan®) on osteoarthritis progression in a rabbit model D Amiel, Ph.D., T Toyoguchi, M.D., Ph.D., K Kobayashi,

L.N. Nwosu, P.I. Mapp, V. Chapman, D.A. Walsh

An in vivo cross-linkable hyaluronan gel with inherent anti-inflammatory properties reduces OA cartilage destruction in female mice subjected to cruciate.

S. Ogawa, Y. Awaga, M. Takashima, A. Hama, A. Matsuda, H. Takamatsu

Protective effect of a new biomaterial against the development of experimental osteoarthritis lesions in rabbit: a pilot study evaluating the intra-articular.

Cartilage MRI T2∗ relaxation time and perfusion changes of the knee in a 5/6 nephrectomy rat model of chronic kidney disease C.-Y. Wang, Y.-J. Peng,

Increase in excitatory amino acid concentration and transporters expression in osteoarthritic knees of anterior cruciate ligament transected rabbits

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the horse C.W. McIlwraith, D.D. Frisbie, C.E.

Angiogenesis in two animal models of osteoarthritis

The impact of forced joint exercise on lubricin biosynthesis from articular cartilage following ACL transection and intra-articular lubricin's effect.

The short-term therapeutic effect of recombinant human bone morphogenetic protein-2 on collagenase-induced lumbar facet joint osteoarthritis in rats

Increase in degraded collagen type II in synovial fluid early in the rabbit meniscectomy model of osteoarthritis E. Lindhorst, M.D., L. Wachsmuth, Ph.D.,

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the rabbit S. Laverty, C.A. Girard, J.M. Williams,

Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model M. Saito, T.

Intra-articular magnesium sulfate (MgSO4) reduces experimental osteoarthritis and nociception: association with attenuation of N-methyl-d-aspartate (NMDA)

D.W. Jackson, M.D., T.M. Simon, Ph.D. Osteoarthritis and Cartilage

Pharmacological effects of novel cross-linked hyaluronate, Gel-200, in experimental animal models of osteoarthritis and human cell lines K. Yoshioka,

Destabilization of the medial meniscus leads to subchondral bone defects and site- specific cartilage degeneration in an experimental rat model H. Iijima,

Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-mediated NF-κBp65-specific siRNA L.X. Chen, Ph.D., L. Lin,

The chemokine receptor CCR5 plays a role in post-traumatic cartilage loss in mice, but does not affect synovium and bone K. Takebe, M.F. Rai, E.J. Schmidt,

Tenascin-C levels in synovial fluid are elevated after injury to the human and canine joint and correlate with markers of inflammation and matrix degradation

Differences in structural and pain phenotypes in the sodium monoiodoacetate and meniscal transection models of osteoarthritis P.I. Mapp, D.R. Sagar,

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the rat N. Gerwin, A.M. Bendele, S. Glasson,

A. Ludin, J.J. Sela, A. Schroeder, Y. Samuni, D.W. Nitzan, G. Amir

B.D. Bomsta, M.S., L.C. Bridgewater, Ph.D., R.E. Seegmiller, Ph.D.

Cyclodextrin polysulphate protects articular cartilage in experimental lapine knee osteoarthritis S. Groeneboer, M.Sc., P. Pastoureau, M.D., Ph.D., E.

A.C. Dang, M.D., A.P. Warren, M.D., H.T. Kim, M.D., Ph.D.

The role of subchondral bone resorption pits in osteoarthritis: MMP production by cells derived from bone marrow A. Shibakawa, M.D., Ph.D., K. Yudoh,

Validation of a 40MHz B-scan ultrasound biomicroscope for the evaluation of osteoarthritis lesions in an animal model Mathieu P. Spriet, D.V.M., Christiane.

The BMP antagonists follistatin and gremlin in normal and early osteoarthritic cartilage: an immunohistochemical study G. Tardif, Ph.D., J.-P. Pelletier,

Quantitative MR T2 measurement of articular cartilage to assess the treatment effect of intra-articular hyaluronic acid injection on experimental osteoarthritis.

E.B. Hunziker, M.D., A. Stähli, D.M.D. Osteoarthritis and Cartilage

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the dog J.L. Cook, K. Kuroki, D. Visco, J.-P.

Alteration of N-glycans related to articular cartilage deterioration after anterior cruciate ligament transection in rabbits T. Matsuhashi, M.D., N.

Osteoarthritis development in novel experimental mouse models induced by knee joint instability S. Kamekura, M.D., K. Hoshi, M.D., Ph.D., T. Shimoaka,

Regeneration of articular cartilage – Evaluation of osteochondral defect repair in the rabbit using multiphasic implants S.R. Frenkel, Ph.D., G. Bradica,

Intra-articular injection of interleukin-4 decreases nitric oxide production by chondrocytes and ameliorates subsequent destruction of cartilage in instability-induced.

Significance of the serum CTX-II level in an osteoarthritis animal model: a 5-month longitudinal study M.E. Duclos, O. Roualdes, R. Cararo, J.C. Rousseau,

Dose–response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study Laurent Galois, M.D., Stéphanie Etienne, M.Sc.,

K. Kuroki, C.R. Cook, J.L. Cook Osteoarthritis and Cartilage

Mevastatin reduces cartilage degradation in rabbit experimental osteoarthritis through inhibition of synovial inflammation Y. Akasaki, M.D., S. Matsuda,

H.L. Reesink, A.E. Watts, H.O. Mohammed, G.D. Jay, A.J. Nixon

Meniscal transection rather than excision increases pain behavior and structural damage in experimental osteoarthritis in mice A.C.R. de Melo Leite,

Histopathological correlation of cartilage swelling detected by magnetic resonance imaging in early experimental osteoarthritis E. Calvo, M.D., I. Palacios,

Developmental failure of the intra-articular ligaments in mice with absence of growth differentiation factor 5 M. Harada, M.D., M. Takahara, M.D., Ph.D.,

Identification of opticin, a member of the small leucine-rich repeat proteoglycan family, in human articular tissues: a novel target for MMP-13 in osteoarthritis

K.L. Caldwell, J. Wang Osteoarthritis and Cartilage

Correlation between the MR T2 value at 4

L. Xu, I. Polur, C. Lim, J.M. Servais, J. Dobeck, Y. Li, B.R. Olsen

Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development C. Pauli, R. Whiteside, F.L.

Preliminary study on diffraction enhanced radiographic imaging for a canine model of cartilage damage C. Muehleman, Ph.D., J. Li, M.D., Z. Zhong, Ph.D.

B.D. Bomsta, M.S., L.C. Bridgewater, Ph.D., R.E. Seegmiller, Ph.D.

The effect of platelet rich plasma combined with microfractures on the treatment of chondral defects: an experimental study in a sheep model G. Milano,

Usefulness of specific OA biomarkers, thrombin-cleaved osteopontin, in the posterior cruciate ligament OA rabbit model S.G. Gao, L. Cheng, C. Zeng, L.C.

I. Gurkan, A. Ranganathan, X. Yang, W. E. Horton, M. Todman, J

Presentation transcript:

Intra-articular injection of the cyclooxygenase-2 inhibitor parecoxib attenuates osteoarthritis progression in anterior cruciate ligament-transected knee in rats: role of excitatory amino acids Y.-H. Jean, M.D., Ph.D., Z.-H. Wen, Ph.D., Y.-C. Chang, M.S., S.-P. Hsieh, M.D., C.-C. Tang, Ph.D., Y.-H. Wang, Ph.D., C.-S. Wong, M.D., Ph.D. Osteoarthritis and Cartilage Volume 15, Issue 6, Pages 638-645 (June 2007) DOI: 10.1016/j.joca.2006.11.008 Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Experimental course. ACLT/S (n=8); ACLT/P (n=9); Naïve/P (n=6); and sham (arthrotomy without ACLT, n=9). Saline injection: intra-articular injection of 0.1ml of saline once a week for 5 consecutive weeks starting at week 8 after surgery. Parecoxib injection: as for the saline group, but using parecoxib (100μg/0.1ml). Osteoarthritis and Cartilage 2007 15, 638-645DOI: (10.1016/j.joca.2006.11.008) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Time-course of joint width changes after surgery. The widths of the bilateral hindlimb knee joints were measured in each rat before, and at 4, 8, 12, 16, and 20 weeks after surgery. The data (mean±s.e.m.) are expressed as the difference in knee width between the values at week 20 after surgery and before surgery in each group. a, P<0.05 compared to the Naïve/P and sham-operated groups; b, P<0.05 compared to the ACLT/S group. Osteoarthritis and Cartilage 2007 15, 638-645DOI: (10.1016/j.joca.2006.11.008) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Histopathological evaluation of cartilage of the medial femoral condyles (stain, Safranin-O/fast green; original magnification, 200×) and synovium (H/E stain, original magnification, 400×) of the knee at week 20 after surgery. (A) ACLT/S group showing surface fibrillation (arrow), chondrocyte loss, and a decrease in Safranin-O/fast green staining intensity, which extends into the radial zone. Cloning of chondrocytes is apparent in the transitional and radial zones. Decrease of Safranin-O/fast green staining in matrix and hyperplasia of chondrocytes in the calcified cartilage layer, and cleft and irregular bony trabeculum between junction of calcified cartilage and subchondral layer are observed. (B) ACLT/P group showing irregularity of the superficial layer of the cartilage (arrow), and mild loss of Safranin-O/fast green staining of the superficial layer of the cartilage. Recovery of proteoglycan and relative normality of chondrocytes in the calcified cartilage layer and smooth bony trabeculum in the subchondral layer are observed. In (C) Naïve/P and (D) sham groups, the superficial layer of cartilage is smooth and no disruption of surface integrity is observed (arrow). The cartilage matrix is well stained with Safranin-O/fast green. Preservation of Safranin-O/fast green staining and chondrocytes in calcified cartilage layer and smooth bony trabeculum is observed in subchondral layer. (E) Synovial membrane from the ACLT/S group, showing hyperplasia of lining cells and hypertrophy of the synovial and subsynovial tissue. Mononuclear cell infiltration of the underlying tissues and a fibrinoid exudate (arrow) are also seen. (F) Synovial membrane from the ACLT/P group shows focal synovitis (arrow) with mild mononuclear cell infiltration. Synovial membrane from the (G) Naïve/P and (H) sham groups shows no synovial lining cell hyperplasia (scale bar=100μm). Osteoarthritis and Cartilage 2007 15, 638-645DOI: (10.1016/j.joca.2006.11.008) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Glutamate and aspartate levels in joint dialysates of ACLT knees from the four groups. EAA levels in the knee joint dialysates were measured at week 20 after surgery. The dialysates were collected for 3h. The mean resting concentration of glutamate and aspartate in synovial fluid dialysates of the contralateral control rats were 2.01±0.15 and 0.33±0.02μM, respectively. The EAA concentrations in the contralateral knee, at week 20, were taken as the basal value (100%) and the glutamate (A) or aspartate (B) levels in the ACLT knee, Naïve/P or sham-operated knee are expressed as the % differences in EAA concentration from the basal 100%. Data are expressed as mean±s.e.m. a, P<0.05 compared to the Naïve/P and sham-operated groups; b, P<0.05 compared to the ACLT/S group. ACLT/S (n=8); ACLT/P (n=9); Naïve/P (n=6); and sham (n=9). Osteoarthritis and Cartilage 2007 15, 638-645DOI: (10.1016/j.joca.2006.11.008) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions