Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge Lars Harder Christensen, PhD,

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Presentation transcript:

Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge Lars Harder Christensen, PhD, Jens Holm, PhD, Gitte Lund, Erik Riise, PhD, Kaare Lund, PhD Journal of Allergy and Clinical Immunology Volume 122, Issue 2, Pages 298-304 (August 2008) DOI: 10.1016/j.jaci.2008.05.026 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Der p 2 epitope mapping. Principle (A), examples (B), and summary (C) of results obtained by means of relative epitope-mapping experiments by using surface plasmon resonance are shown. Principle (D), example (E), and summary (F) of results obtained by means of epitope-mapping experiments with rDer p 2 variants by using surface plasmon resonance are shown. G and H, Three- and 2-dimensional maps of Der p 2 epitopes bound by the rIgE clones. Source of Der p 2 structure: Derewenda et al13; PDB ID, 1KTJ. Journal of Allergy and Clinical Immunology 2008 122, 298-304DOI: (10.1016/j.jaci.2008.05.026) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE affinities for rDer p 2. A-C, Examples of affinity determinations of rIgE clones binding rDer p 2 with high, medium, and low affinity, respectively, as obtained by using surface plasmon resonance experiments. Only the relevant part from where rDer p 2 is injected is shown. D, Summary of affinities obtained of all rIgE clones (color coded according to the epitope specificities marked in Fig 1, G and H). Journal of Allergy and Clinical Immunology 2008 122, 298-304DOI: (10.1016/j.jaci.2008.05.026) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Basophil degranulation at different concentrations of total rIgE. FACS results of human basophils sensitized with total rIgE concentrations of 3, 1, 0.2, 0.04, 0.008, and 0.0016 μg/mL, respectively, are shown. The composition of the rIgE mixture was kept constant with 20% rDer p 2–specific rIgE consisting of equimolar quantities (6.67% each) of 3 rIgE clones (G, H10, and H12) recognizing nonoverlapping Der p 2 epitopes and 80% non–Der p 2–specific rIgE (anti-tox). Upper left, Schematic representation of Der p 2 epitope specificity and affinity of rIgE clones G, H10, and H12; analog to Figs 1, H, and 2, D. H, High affinity; M, medium affinity; L, low affinity. Journal of Allergy and Clinical Immunology 2008 122, 298-304DOI: (10.1016/j.jaci.2008.05.026) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Basophil degranulation at different concentrations of Der p 2–specific rIgE relative to non–Der p 2–specific rIgE. FACS results of human basophils sensitized with different concentrations of Der p 2–specific rIgE (srIgE) relative to non–Der p 2–specific rIgE (non-srIgE) are shown. The composition of the rIgE mixtures consisted of equimolar quantities of 3 rIgE clones (E, H10, and H12) recognizing nonoverlapping Der p 2 epitopes and variable concentrations of non–Der p 2–specific rIgE (anti-tox). The total rIgE concentration was kept constant at 1 μg/mL. Journal of Allergy and Clinical Immunology 2008 122, 298-304DOI: (10.1016/j.jaci.2008.05.026) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Basophil degranulation at different ratios between 2 Der p 2–specific rIgE clones. FACS results of human basophils sensitized with different ratios of 2 Der p 2–specific rIgE clones (E and H12) are shown. All other parameters were kept constant; that is, the total concentration of rIgE was 1 μg/mL, and the relative concentrations were 20% Der p 2–specific to 80% non–Der p 2–specific rIgE. Journal of Allergy and Clinical Immunology 2008 122, 298-304DOI: (10.1016/j.jaci.2008.05.026) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Basophil degranulation at different rIgE affinity combinations. FACS results of human basophils sensitized with different combinations of 2 rIgE clones having different affinities for rDer p 2 are shown. All other parameters were kept constant; that is, rIgE clones were directed against the same 2 epitope clusters, the total IgE concentration was 1 μg/mL, the relative concentrations were 20% Der p 2–specific to 80% non–Der p 2–specific rIgE, and equimolar concentrations of the 2 Der p 2–specific rIgE clones were used. H, High affinity; M, medium affinity; L, low affinity. Journal of Allergy and Clinical Immunology 2008 122, 298-304DOI: (10.1016/j.jaci.2008.05.026) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 7 Basophil degranulation at different rIgE clonalities. FACS results of human basophils sensitized with combinations of 1, 2, or 3 rIgE clones binding nonoverlapping Der p 2 epitopes are shown. All other parameters were kept constant; that is, the total IgE concentration was 1 μg/mL, the relative concentrations were 20% Der p 2–specific to 80% non–Der p 2–specific rIgE, and equimolar concentrations of the rDer p 2–specific rIgE clones were used. Journal of Allergy and Clinical Immunology 2008 122, 298-304DOI: (10.1016/j.jaci.2008.05.026) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions