Karthi Sivaraman Department of Molecular and Microbiology,

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Presentation transcript:

Role of Nucleotide Polymorphisms in Evolution of Staphylococcal Minimal Genome Karthi Sivaraman Department of Molecular and Microbiology, University of Central Florida

The common minimal genome of Staphylococcus aureus Common minimal genome (CMG) Set of orthologous genes present in all the strains of a given species The staphylococcal CMG (14 strains) 2001 genes 765 genes of known function 1236 Hypothetical proteins

Non-Synonymous SNP distribution in the S. aureus CMG

Classification of Genes with high SNP variation – Top 5% Majority of the genes belong to pathogenicity (20) / biofilm (4) formation group Total genes in category = 199 Total genes for which function could be assigned = 45

Classification of Genes with zero non-synonymous SNP Majority of the genes belong to metabolism and macromolecule biosynthesis groups Total genes in category = 637 Total genes for which function could be assigned = 268

NIH Grant 5R01-AI060753 to Alexander M Cole Conclusions Pathogenesis related genes in S. aureus minimal genome accrue more SNPs than do house keeper genes Evolution of S. aureus pathogenicity involves both large scale horizontal gene transfer as well as micro scale changes in the minimal genome. Acknowledgements Dr. Alexander Cole, Nitya Venkataraman, and Conference Organizers Funding NIH Grant 5R01-AI060753 to Alexander M Cole