H3K9me2 and G9a levels decrease during adipogenesis.

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H3K9me2 and G9a levels decrease during adipogenesis. H3K9me2 and G9a levels decrease during adipogenesis. (A) ChIP analyses of H3K4me3, H3K9me2 and H3K27me3 on PPARγ1 and PPARγ2 promoters before (day 0) and after (day 6) 3T3‐L1 adipogenesis. The schematic of PPARγ1 and PPARγ2 promoters and the locations of Taqman probes for PCR quantitation of ChIP are shown at the top. Wnt10a promoter serves as a control. (B) ChIP‐Seq profiles of H3K9me2 on PPARγ locus (highlighted) before (day 0) and after (day 7) 3T3‐L1 adipogenesis. (C) qRT–PCR analysis of PPARγ and G9a expression before and after 3T3‐L1 adipogenesis. (D) Western blot analysis of G9a, PPARγ and H3K9me2 levels during 3T3‐L1 adipogenesis. The 54.5‐kDa PPARγ1 and 57.5‐kDa PPARγ2 bands as well as the 30‐kDa and 42‐kDa C/EBPα isoforms are indicated. The p85α subunit of phosphoinositol‐3‐phosphate kinase is used as a loading control. (E) Western blot analysis of protein levels in preadipocytes and adipocytes isolated from mouse inguinal white adipose tissue.Source data for this figure is available on the online supplementary information page. Lifeng Wang et al. EMBO J. 2013;32:45-59 © as stated in the article, figure or figure legend