gRAD (generic Rapid Assay Device) New Product Introduction gRAD (generic Rapid Assay Device) Dina Gonçalves December 2015

Outline What, How and Why? Where and how to sell gRAD What is gRAD? How does gRAD works? Why to use gRAD? Products portfolio Where and how to sell gRAD Customers References Market strategies Competing technologies/approaches Unique selling points Marketing Material Introductory special offer to distributors

What, How and Why?

What is gRAD? Standard lateral flow (test-specific) gRAD (generic) sample pad sample pad test line biotin-binding protein specific antibodies control line anti-mouse / rabbit / goat antibodies generic antibodies conjugate pad gRAD (generic)

What is gRAD? Standard lateral flow (test-specific) flow

What is gRAD? Standard lateral flow (test-specific) Assay-specific Defined antibodies in the strip (detection and conjugated to gold) Biological recognition events on strip By custom outsourced development or requiring especial printing equipment

How does gRAD works? gRAD (generic) 1 test line + 1 control line sample pad test line biotin-binding protein control line anti-mouse / rabbit / goat antibodies 1 test line + 1 control line Serum, plasma, urine, solutions of antigen, solid material dissolved in diluent Consistency of test line – CV 2-5% 50 pg/ml – 500 ng/ml, on device Monoclonal antibodies, polyclonal affinity-purified, provided by the user Mouse, rabbit or goat antibodies

antigen-antibodies complex How does gRAD works? detection antibody conjugated to gold antigen-antibodies complex biotinylated capture antibody antigen flow gRAD (generic) B B B

How does gRAD works? gRAD (generic) B B B

How does gRAD works? Developing and optimizing an assay Running an optimized assay Producing gold conjugates Finding the analyte concentrations giving a signal (visible test line) Optimization of the capture reagent Optimization of the detection reagent

How does gRAD works? Important points to consider Gold conjugation Testing different capture antibody concentrations Low antibody concentration  Higher sensitivity, but slower and less precise assay High antibody concentration  Lower signal intensity, but faster and more precise assay Testing different gold conjugated antibody volumes Increasing volume detection Ab  Test line signal increases, but also the background

How does gRAD works? Developing and optimizing an assay Running an optimized assay Pre-test user steps Conjugation Mixing components Test user steps Dipping strip in reaction mix Evaluation of test line intensity

How does gRAD works? PRE-TEST USER STEPS TEST USER STEPS Test Reagent Test Mixture TEST USER STEPS

Why to use gRAD? Easier and faster in-house development of rapid assays Multiple uses and applications Simple procedures No need for specialized equipment Development of niche applications Possible quantification without need of a readers and expensive software Keeping internal knowledge Different levels of complexity

Products Portfolio REF PRODUCT LIST PRICE gRAD(1) - KIT gRAD OneDetection - Kit 900 € gRAD(1) - 120 gRAD OneDetection – 120 strips 480 € NGIB18-1 Naked Gold Conjugation Kit (20 nm & 40 nm) NGIB18-2 Naked Gold Conjugation Kit (20 nm) NGIB18-3 Naked Gold Conjugation Kit (40 nm) SDB50 Sample Dilution Buffer 10 € The complete solution to start developing gRAD assays Perfect for testing new ideas and running optimized assays Optimized gold conjugation kit Optimized buffer to enhance gRAD assays performance

Products Portfolio gRAD(1) - KIT gRAD(1) - 120 NGIB18 SDB50 calibration card

Where and how to sell?

Customers Research and Development in: Veterinarian applications Livestock Companion animals Feed contamination control Milk and meet quality control Diagnostics, human Tropical diseases Cancer Biomarkers pre-screening Quality control, pharma and biologics …and more…

References Foot and mouth disease Yang et al. (2015) Development of a multiplex lateral flow strip test for foot-and-mouth disease virus detection using monoclonal antibodies. J. Virol. Methods 221:119-26 Yang et al. (2013) Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic Rapid Assay Device. Virol. J. 10:125.

Competing technologies/approaches Outsourcing custom development Development in-house from scratch Other rapid assays technologies

Marketing strategies Materials Mailings Video Printed material Campaigns Entry offer / trial - 20 strips for free Partner Program (for high-impact academic institutions) Demos / Conferences 2016 Custom service / offers Discount matrix for bulk orders

Marketing material Website http://www.bioporto.com/Products/Product-by-category/Lateral-flow-1.aspx Video Printed Material

Unique selling points Qualitative Semi-Quantitative Open and Flexible Platform Total customization Possible Quantitative Assays Time and Money Saving Easier and faster Ready-to-use kit format Reactions in solution Patented Technology (WO2009062506 A1)

Introductory special offer to distributors Dec 2015 ORDER in December 2015 Get EXTRA DISCOUNT on gRAD products purchase 2016 Total purchase target value in 2016 % purchase value in gRAD products 2017 Guaranteed extra discount of 5% on gRAD products (3 distributors awarded)

Extra discount December 2015 Any purchase (min 1 x gRAD(1) – KIT + other product(s) at least the same value) Extra* 10 % on gRAD 5 x gRAD(1) – KIT or 3 x gRAD(1) – KIT + 4 gRAD(1) – 120 or 2 x gRAD(1) – KIT + 6 gRAD(1) – 120 Extra* 20 % on gRAD >10 x gRAD(1) – KIT or 5 x gRAD(1) – KIT + 10 gRAD(1) – 120 Extra* 30 % on gRAD * On top of distributor discount (the same you have for Abs/ELISAs)

Qualification for extra discount 2017 GROUP MINIMUM PURCHASE TARGET Group A (small distributors) 5.000 € Group B (medium distributors) 35.000 € Group C (large distributors) 100.000 €

Making gRAD a success together! Make all your questions! Return feedback from customers Keep close contact to provide excellent support Share special requests from customers, ideas for improvement and new gRAD versions Share feedback on interest for quantitative setup Co-market gRAD with other products (antibodies, matched pairs, etc..) Share your ideas to reach new customers

Thank you. BioPorto Diagnostics A/S Tuborg Havnevej 15, st. DK-2900 Hellerup Denmark Phone (+45) 4529 0000 E-mail info@bioporto.com Web www.bioporto.com LinkedIn https://www.linkedin.com/company/bioporto-diagnostics-a-s Dina Goncalves, PhD Key Account Manager dg@bioporto.com