Enzyme Annotation with Chemical Tools

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Presentation transcript:

Enzyme Annotation with Chemical Tools Ariel G. Herman, Brent R. Stockwell Chemistry & Biology Volume 13, Issue 10, Pages 1013-1014 (October 2006) DOI: 10.1016/j.chembiol.2006.10.002 Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 1 Scheme for Biochemical Characterization of KIAA1363 Using Activity-Based Protein Profiling and Metabolic Profiling The use of ABPP identified a novel enzyme, KIAA1363, upregulated in cancer cells, but not in normal cells. Competitive ABPP facilitated the development of a small-molecule inhibitor of KIAA1363, AS115. Metabolic profiling was performed with AS115 to identify the endogenous substrate of KIAA1363. Further small-molecule studies with AS115 allowed for the biochemical annotation of KIAA1363 in a near-native system. Chemistry & Biology 2006 13, 1013-1014DOI: (10.1016/j.chembiol.2006.10.002) Copyright © 2006 Elsevier Ltd Terms and Conditions