Key points: summarized from chapter 14

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Presentation transcript:

Key points: summarized from chapter 14 RNA processing

Outline: we will focus on mRNA processing Overview of the Central Dogma Link DNA to RNA transcription to proteintranslation What is a codon? Gene organization Introns and exons What really is a gene? Steps of pre-mRNA processing Eukaryotes versus prokaryotes

Gene Organization: Is DNA and RNA co-linear?

3 steps of mRNA processing occurs in the nucleus of eukaryotes 5’ CAP Poly-A tail Splicing

The 5’Cap

The Poly-A Tail

Cleavage by endonuclease

Basic steps of poly-Adenylation

Splicing Spliceosome Requires RNA + proteins Self splicing Only RNA! Function as enzymes Self splicing Only RNA! Function as enzymes!

Spliceosome Meet the spliceosome

Splicesome: 3 components are required Note some important aspects of the 5’ and 3’ ends 5’ phosphodiester of G 2’ OH of A lariat

Where does splicing occurs?

Self-Splicing mRNA’s

Common characteristic of self-splicing mRNA’s

Comments on Alternative processing methods First: What are the methods of mRNA processing discussed so far? 5’ CAP 3’ Poly A Tail Intron Splicing

Alternative processing methods Alternative splicing Multiple 3’cleavage sites RNA editing

And alternative splicing example Thryoid BRAIN

Silencing or interfering RNAs siRNA miRNA

Onto translation chapter 15