Reply to Rampoldi et al. Kidney International Yuqing Chen, Daniel T. O’Connor  Kidney International  Volume 84, Issue 2, Pages 411-413 (August 2013) DOI: 10.1038/ki.2013.164 Copyright © 2013 International Society of Nephrology Terms and Conditions

Figure 1 Uromodulin (UMOD) haplotypes in the promoter region. (a) Promoter sense strand: multiple alignment of fragments inserted into the promoter/reporter vector and the reference sequence. Promoter/reporter vectors (pGL3-Basic) containing UMOD promoter sense-strand inserts were sequence-verified. Two of the clones with the most common haplotype (TAT) and the reference sequence (NM_003361) are presented. Multiple alignment figures were shorted. Numbers shown on the right are nucleotide positions. Positions of the three proximal promoter single-nucleotide polymorphisms (sense strand: rs13333226 (T>C), rs6497476 (A>G), and rs4293393 (T>C)) are also shown. The UMOD sense-strand reference sequence (RefSeq NM_003361) was obtained from the UCSC genome database browser at http://www. genome.ucsc.edu/cgi-bin/hgGateway. (b) UMOD promoter alleles and haplotypes: statement of sense/antisense strands and clarification of nomenclature. Left: Sense-strand haplotypes (most frequent and mutant) in the pGL3-Basic vector (upon sequence verification). Sense strand: rs13333226 (T>C), rs6497476 (A>G), and rs4293393 (T>C). Right: Haplotype names used in our text and figures. The strands that gave rise to this nomenclature are as follows: rs13333226 (A>G, antisense), rs6497476 (T>C, antisense), and rs4293393 (T>C, sense). Kidney International 2013 84, 411-413DOI: (10.1038/ki.2013.164) Copyright © 2013 International Society of Nephrology Terms and Conditions