Anti-CD20 monoclonal antibody treatment in 6 patients with therapy-refractory chronic graft-versus-host disease by Marijke R. Canninga-van Dijk, Hanneke M. van der Straaten, Rob Fijnheer, Cornelus J. Sanders, Jan G. van den Tweel, and Leo F. Verdonck Blood Volume 104(8):2603-2606 October 15, 2004 ©2004 by American Society of Hematology

Clinical and histopathological improvement. Clinical and histopathological improvement. (A) Patient no. 4 before therapy. (B) Patient no. 4 after therapy. (C) Patient no. 1 before therapy (H&E stain; magnification × 200). Dyskeratotic cells in the epidermis with a mild lymphocytic infiltrate. (D) Patient no. 1 after therapy (H&E stain; magnification × 200). Improvement of the epidermal features. (E) Patient no. 6 before therapy (Sirius red stain; magnification × 200). Compact, severely thickened collagen in the superficial dermis. (F) Patient no. 6 after therapy (Sirius red stain; magnification × 200). The collagen is less compact. (G) Patient no. 6 before therapy (CD68 stain; magnification × 200). A few macrophages are present in the superficial dermis. The arrows in panel G indicate CD68-positive macrophages. (H) Patient no. 6 after therapy (CD68 stain; magnification × 200). The macrophages have disappeared. The microscope used was a Nikon Eclipse E800M with a Nikon Plan apo 20 × objective. The camera used was a digital Nikon FDX-35. Acquisition software and processing software were Nikon ACT-1 and Photoshop, respectively. Marijke R. Canninga-van Dijk et al. Blood 2004;104:2603-2606 ©2004 by American Society of Hematology