Welcome! X-Ray Facility, 410-413 Kasha Laboratory The presentation is intended to showcase the capabilities of the Kasha Laboratory’s Macromolecular X-Ray Diffraction Facility. It will feature approximately twenty slides and will last one half hour. X-Ray Facility, 410-413 Kasha Laboratory Institute of Molecular Biophysics Florida State University Tallahassee, FL 32306-4380 www.sb.fsu.edu/~xray ã Thayumanasamy Somasundaram, 2003-2004

X-Ray Facility Orientation History of the Facility Facility Resources Requirements Data Collection Data Processing & Manuals Archiving & Summary Contact & Information This slide provides an overview of the presentation. Each item will be elaborated later in one or multiple slides. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

History of the Facility-1 Important Dates Core facility planned in 1990-92 Facility Director hired Feb 1993 Construction June – Sep 1993 Two generators & Image Plate (IP) detector in January 1994 Elliott generator in 1995 This slide shows the time line of the IMB X-Ray Facility. Important additions and modifications are shown. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

History of the Facility-2 Important Dates Cryo capabilities in 1996 Crystal chamber in 1999 Confocal mirror in Spring 2000 CCD w/ confocal mirror in Fall 2000 CryoCooler 700 w/ auto-refill in Fall 2000 Additional Linux machines in 2000 Complete renovation during 2002-03 This continuation slide shows the time line of the IMB X-Ray Facility. Important additions and modifications are shown. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Facility Resources & Accessories Three Copper rotating anode generators Two automated area detectors Two confocal & one total reflection mirrors Two cryo coolers One automated liquid Nitrogen refiller Linux and Windows NT computers Full time Faculty/Staff (State-line) This slide gives the overview of the X-Ray Facility Resources ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Resources-1 Generators Two Rigaku H2R 5.4 kW generators One Elliott GX-30 3.0 kW generator Area detector-1 (IP) R-Axis IIc (1994) w/ confocal blue mirror 65-450 mm sample-detector distance 0-25 degree non-zero 2-theta capability Oxford Cryostream 600 cold head This and next slides show the core instruments of the X-Ray Facility and a brief description of their capabilities. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Resources-2 Area detector-2 (CCD) Mar165 CCD w/ confocal purple mirror 35-380 mm sample-CCD distance Limited non-zero 2-theta capability Oxford Cryostream 700 cold head Long & short Supper (total-reflection) mirrors Stand-alone IP Bio Unit I Analytical Lab This and previous slides show the core instruments of the X-Ray Facility and a brief description of their capabilities. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Accessories-1 Leica stereo microscope w/ Moticam digital camera Nikon Optiphot microscope w/ analog camera Stereo zoom microscopes DVD-R & +R writers DDS-3 & -4 tape drives FireWire (Maxtor: 60 & 80 GB) drives Windows NT and five Linux computers This and next slides show the auxiliary instruments & computational resources of the X-Ray Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Accessories-2 Cold room for 4°C crystallization set-up Room for ambient crystallization set-up Crystallization chamber Temperature & humidity monitoring Dry Cryo Shipper Long-term sample storage Laptop for synchrotron trips This and previous slides show the auxiliary instruments & computational resources of the X-Ray Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Requirements Member of Structural Biology Program Radiation safety training Computer account (UNIX/LINUX/NT) Body and/or finger radiation badge No cost to use Users bring consumables: CryoLoops, Capillaries, Tape and DVD media This slide explains who is eligible to use X-Ray Facility and the cost associated with the usage. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Data Collection-1 Sample mounting decision Capillary or loop mounting? Data collection temperature Ambient or cryo? If cryo, cryo protection selection Grow or soak crystal? Detector decision Image plate or CCD detector? This and next slides address the decisions the user has to make before using the X-Ray Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Data Collection-2 Screening or regular data collection? Improvement or brand-new crystal? Resolution decision Low, moderate, or high-resolution? 2-theta angle Cell dimension/detector distance? This and next slides address the decisions the user has to make before using the X-Ray Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Data Collection Procedure Check the schedule at: www.sb.fsu.edu/~soma/XraySchedules/schfrmset.html Request time by e-mail: soma@sb.fsu.edu by phone: 644-6448 Priority given for samples in the beam Priority given to unstable samples Flexible schedule & co-operation is valued This slide explains the general procedure requesting time for collecting x-ray diffraction data at the Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Data Processing Procedure Collect preliminary data & auto index If successful, complete data collection If not, modify procedure and try Index, integrate, scale full data set Repeat integration & scaling, if needed Validate and compare Archive & transfer files This slide explains the general scheme of data processing during a typical data collection run at the Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Archiving Data is the responsibility of the “USER” Available archiving resources: DDS-3 & DDS-4 tape drives DVD-R & +R writers Users bring the media Facility archives data (last resort) Computer Resources may be helpful This slide shows the available resources for archiving (back-up) the data collected at the Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Manuals & Documents Online version at Paper version in 410 KLB (XRF) http://www.sb.fsu.edu/~xray/Manuals/soft_man.html Paper version in 410 KLB (XRF) Program authors’ website Manufacturer’s website IMB resource pages Lab protocols and procedures This slide shows the various manuals, documentation and other resources available to the user for a fruitful experience at the Facility. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Summary State of the art facility Excellent scientific/support staff: Computer Resources, Protein Expression Facility, Physical Biochem Facility, Electronics shop, & Machine shop Keep it clean and in working order Report problems to Soma (4-6448) Summary of the Facility and other Resources at IMB. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Contacts & Information-1 Data collection, processing, scheduling: Thayumanasamy Somasundaram [SOMA] Ph: 644-6448 | E-mail: soma@sb.fsu.edu www.sb.fsu.edu/~soma | www.sb.fsu.edu/~xray XRF (KLB 410-413) | 645-1333 Computer related: Mike Zawrotny Ph: 644-0069 | zawrotny@sb.fsu.edu This and next slides show how to contact people for more information. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Contacts & Information-2 Electronics: Rick Wang Ph: 645-3309 | wang@sb.fsu.edu Machine Shop | Wood shop: Wade Baggett or Ed Kirkland Ph: 644-1419 | 645-1338 This and previous slides show how to contact people for more information. ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

IP & CCD Detectors IMB (2001) Picture Gallery IP and CCD detectors along with rotating anode generators in pre-renovated Institute of Molecular Biophysics Building. Circa 2001. IP & CCD Detectors IMB (2001) ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

IP & CCD Detectors DLC (2002) Picture Gallery IP and CCD detectors along with rotating anode generators in Dittmer Laboratory of Chemistry (temporary location). Circa 2002. IP & CCD Detectors DLC (2002) ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

IP & CCD Detectors KLB (2004) Picture Gallery IP and CCD detectors along with rotating anode generators in renovated Kasha Laboratory, Institute of Molecular Biophysics Building. Circa 2004. IP & CCD Detectors KLB (2004) ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018

Crystal Room & Computer Room (2004) Picture Gallery Room-temperature crystal set-up room (KLB 412) and data-processing computer room (KLB 410A) in renovated Kasha Laboratory, Institute of Molecular Biophysics Building. Circa 2004. Crystal Room & Computer Room (2004) ã Thayumanasamy Somasundaram, 2003-2004 11/21/2018