A production system to generate reference genetic profiles from Buccal Swab cells on FTA® cards  L. Baron, E. Suzanne, A. Calletier, C. Pardo, L. Pène  Forensic Science International: Genetics Supplement Series  Volume 3, Issue 1, Pages e560-e561 (December 2011) DOI: 10.1016/j.fsigss.2011.10.021 Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Sample lysis worflow. (A) The user scans the evidence barcode as well as a single FluidX Easytrack 2D Barcoded 2ml tube. A ABI PrepFiler Lysep™ is inserted in this FluidX tube. The sample (swab, cigarette butt or other) is inserted in the Lysep column. Lysis buffer is 505μl of PrepFiler Lysis buffer (500μl PrepFiler Lysis buffer+5μl DTT). (B) The Lysep lid is closed and the tube is placed in the 24 tube rack. (C) Lysis incubation 70°C/40min. (D) After lysis the racks are centrifuged for 10min at 2000×g. (E) Remove the Lysep™ columns. (F) The racks are placed on the Hamilton Microlab STARlet for DNA extraction using the standard high volume PrepFiler™ protocol (Fig. 2). Forensic Science International: Genetics Supplement Series 2011 3, e560-e561DOI: (10.1016/j.fsigss.2011.10.021) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Deck layout of the forensic starlet workstation. Forensic Science International: Genetics Supplement Series 2011 3, e560-e561DOI: (10.1016/j.fsigss.2011.10.021) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions