Volume 57, Issue 6, Pages 1111-1114 (June 2010) Focal Laser Ablation for Prostate Cancer Followed by Radical Prostatectomy: Validation of Focal Therapy and Imaging Accuracy  Uri Lindner, Nathan Lawrentschuk, Robert A. Weersink, Sean R.H. Davidson, Orit Raz, Eugen Hlasny, Deanna L. Langer, Mark R. Gertner, Theodorus Van der Kwast, Masoom A. Haider, John Trachtenberg  European Urology  Volume 57, Issue 6, Pages 1111-1114 (June 2010) DOI: 10.1016/j.eururo.2010.03.008 Copyright © 2010 European Association of Urology Terms and Conditions

Fig. 1 Fixed 3-mm-thick prostate slices before paraffin embedding. White arrow points toward laser fiber placement; red arrows indicate grossly visible ablated area. European Urology 2010 57, 1111-1114DOI: (10.1016/j.eururo.2010.03.008) Copyright © 2010 European Association of Urology Terms and Conditions

Fig. 2 Vital staining of the prostate for cytokeratin 8. (A) Whole-mount vital stain of the prostate. Arrows indicate position of the laser fibers. (B) Zoom in view using ScanScope XT slide scanning systems. No vital cells are evident between the laser fibers. European Urology 2010 57, 1111-1114DOI: (10.1016/j.eururo.2010.03.008) Copyright © 2010 European Association of Urology Terms and Conditions

Fig. 3 Ablation extending to the prostatic capsule. (A) Ablation zone extends all the way to the posterior border. Arrows mark where the laser fibers were positioned. No viable cells were seen inside the 18-mm ablated area. (B) Ablation zone extending posteriorly and laterally. Arrows mark the position of the laser fibers. European Urology 2010 57, 1111-1114DOI: (10.1016/j.eururo.2010.03.008) Copyright © 2010 European Association of Urology Terms and Conditions

Fig. 4 Registration of ablation in magnetic resonance (MR) imaging and pathology. (A) MR image with the damage contouring. Black arrows indicate where the laser fibers were placed. (B) Same pathologic slice. Black arrows indicate where the laser fibers were placed. Red arrows indicate where the fiducial markers were placed. European Urology 2010 57, 1111-1114DOI: (10.1016/j.eururo.2010.03.008) Copyright © 2010 European Association of Urology Terms and Conditions