Increased endogenous angiogenic response and hypoxia-inducible factor-1α in human critical limb ischemia Teik K. Ho, MRCS, Vineeth Rajkumar, MSc, Markella.

Slides:

Advertisements

Similar presentations

Dermal tissue fibrosis in patients with chronic venous insufficiency is associated with increased transforming growth factor-β1 gene expression and protein.

Advertisements

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Chung S. Lim, MRCS, PhD, Serafim Kiriakidis, PhD, Ewa M

High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Adeno-associated virus serotype 9-mediated overexpression of extracellular superoxide dismutase improves recovery from surgical hind-limb ischemia in.

The association of carotid plaque inflammation and Chlamydia pneumoniae infection with cerebrovascular symptomatology Elias A. Kaperonis, MD, Christos.

Chung S. Lim, MRCS, PhD, Serafim Kiriakidis, PhD, Ewa M

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia Geoffrey O. Ouma,

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis April E. Nedeau, MD,

The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.

An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.

Hypercholesterolemia Impairs the Myocardial Angiogenic Response in a Swine Model of Chronic Ischemia: Role of Endostatin and Oxidative Stress Munir Boodhwani,

Potential role of erythropoietin receptors and ligands in attenuating apoptosis and inflammation in critical limb ischemia Dhiraj Joshi, MD, MRCS, David.

Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.

Myriam Martin, PhD, Anders Gottsäter, MD, PhD, Peter M

Markers of instability in high-risk carotid plaques are reduced by statins Hagen Kunte, MD, Nicola Amberger, MD, Markus Alexander Busch, MD, MPH, Ralph-Ingo.

Volume 71, Issue 3, Pages (February 2007)

Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection Lixin Wang, MD, PhD, Jing Zhang, MD, PhD,

Combination of stromal-derived factor-1α and vascular endothelial growth factor gene- modified endothelial progenitor cells is more effective for ischemic.

Increased vascularization of shoulder regions of carotid atherosclerotic plaques from patients with diabetes Fredrik J. Olson, PhD, Sofia Strömberg,

Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation Pascal Jeanmonod, MD, Matthias.

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia Sébastien Déglise, MD, David Martin, MS, Hervé.

Differential expression of elastin assembly genes in patients with Stanford Type A aortic dissection using microarray analysis Bernice L.Y. Cheuk, PhD,

Cellular and molecular mechanism regulating blood flow recovery in acute versus gradual femoral artery occlusion are distinct in the mouse Yagai Yang,

Perigraft vascularization and incorporation of implanted Dacron prostheses are affected by rifampicin coating Mohammed R. Moussavian, MD, Matthias W.

Metformin stimulates ischemia-induced revascularization through an eNOS dependent pathway in the ischemic hindlimb mice model Noriko Takahashi, MD, Rei.

Potential role of erythropoietin receptors and ligands in attenuating apoptosis and inflammation in critical limb ischemia Dhiraj Joshi, MD, MRCS, David.

Inducible nitric oxide synthase is present in human abdominal aortic aneurysm and promotes oxidative vascular injury Jian Zhang, MD, Jan Schmidt, MD,

Proinflammatory role of stem cells in abdominal aortic aneurysms

Relative importance of aneurysm diameter and body size for predicting abdominal aortic aneurysm rupture in men and women Ruby C. Lo, MD, Bing Lu, MD,

Association of intraluminal thrombus in abdominal aortic aneurysm with local hypoxia and wall weakening David A. Vorp, PhDa,b,c, Paul C. Lee, MDa, David.

Nanoparticle-mediated endothelial cell-selective delivery of pitavastatin induces functional collateral arteries (therapeutic arteriogenesis) in a rabbit.

Structural analysis and magnetic resonance imaging predict plaque vulnerability: A study comparing symptomatic and asymptomatic individuals Zhi-Yong.

Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath Vikram Vijayan,

Tissue factor pathway inhibitor-2 is induced by fluid shear stress in vascular smooth muscle cells and affects cell proliferation and survival Johan.

Mechanosensitive transient receptor potential vanilloid type 1 channels contribute to vascular remodeling of rat fistula veins Yih-Sharng Chen, MD, PhD,

Leptin receptor is elevated in carotid plaques from neurologically symptomatic patients and positively correlated with augmented macrophage density Jacob.

Therapeutic site selection is important for the successful development of collateral vessels Ayako Nishiyama, MD, PhD, Hiroyuki Koyama, MD, PhD, Tetsuro.

Markers of instability in high-risk carotid plaques are reduced by statins Hagen Kunte, MD, Nicola Amberger, MD, Markus Alexander Busch, MD, MPH, Ralph-Ingo.

Autophagy-mediated stress response in motor neurons after hypothermic spinal cord ischemia in rabbits Satoshi Fujita, MD, Masahiro Sakurai, MD, PhD,

Bronchial mucosal IFN-α/β and pattern recognition receptor expression in patients with experimental rhinovirus-induced asthma exacerbations Jie Zhu,

Protection by 20-5,14-HEDGE Against Surgically Induced Ischemia Reperfusion Lung Injury in Rats Irshad Ali, PhD, Stephanie Gruenloh, BS, Ying Gao, MS,

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Prolonged increases in vein wall tension increase matrix metalloproteinases and decrease constriction in rat vena cava: Potential implications in varicose.

Characterization of primary and restenotic atherosclerotic plaque from the superficial femoral artery: Potential role of Smad3 in regulation of SMC proliferation

Aortic wall cell proliferation via basic fibroblast growth factor gene transfer limits progression of experimental abdominal aortic aneurysm Katsuyuki.

Jayer Chung, MD, J. Gregory Modrall, MD, R. James Valentine, MD

Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections Rumin He,

Clinical effect of wound depth in critical limb ischemia with tissue loss after endovascular treatment Norihiro Kobayashi, MD, Keisuke Hirano, MD, Masatsugu.

Carotid plaque instability and ischemic symptoms are linked to immaturity of microvessels within plaques Benjamin J. Dunmore, PhD, Mark J. McCarthy,

Role of matrix metalloproteinases 1, 2, and 9 and tissue inhibitor of matrix metalloproteinase-1 in chronic venous insufficiency Satoshi Saito, MD, Matthew.

Kim G. Smolderen, PhD, Aline J

Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia Masatake Katsu, MD, Hiroyuki.

Protease-Activated Receptor 2, a Receptor Involved in Melanosome Transfer, is Upregulated in Human Skin by Ultraviolet Irradiation Glynis Scott, Cristina.

Dermal tissue fibrosis in patients with chronic venous insufficiency is associated with increased transforming growth factor-β1 gene expression and protein.

In vitro differences between smooth muscle cells derived from varicose veins and normal veins Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.

Human angiopoietin gene expression is a marker for severity of pulmonary hypertension in patients undergoing pulmonary thromboendarterectomy Patricia.

Endoplasmic reticulum stress induced in motor neurons by transient spinal cord ischemia in rabbits Masahiro Sakurai, MD, PhD, Goro Takahashi, MD, PhD,

Autophagy-mediated stress response in motor neuron after transient ischemia in rabbits Hironori Baba, MD, Masahiro Sakurai, MD, PhD, Koji Abe, MD, PhD,

Autologous skeletal myoblasts transduced with a new adenoviral bicistronic vector for treatment of hind limb ischemia Muhammad I. Niagara, MSc, Husnain.

Long-term results of a randomized controlled trial on ultrasound-guided foam sclerotherapy combined with saphenofemoral ligation vs standard surgery for.

Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study Wangde Dai,

Deep vein thrombosis resolution is impaired in diet-induced type 2 diabetic mice Fatiha Bouzeghrane, PhD, Xiaochun Zhang, MD, BSc, Guylaine Gevry, BSc,

Diastolic function predicts survival after renal revascularization

Virtual reality simulation objectively differentiates level of carotid stent experience in experienced interventionalists Isabelle Van Herzeele, MD,

Activated protein C-protein C inhibitor complex: A new biological marker for aortic aneurysms Tilo Kölbel, MD, Karin Strandberg, MD, PhD, Ingrid Mattiasson,

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Family history of aortic aneurysm is an independent risk factor for more rapid growth of small abdominal aortic aneurysms in Japan Atsushi Akai, MD,

Presentation transcript:

Increased endogenous angiogenic response and hypoxia-inducible factor-1α in human critical limb ischemia Teik K. Ho, MRCS, Vineeth Rajkumar, MSc, Markella Ponticos, PhD, Patricia Leoni, PhD, Dame Carol M. Black, PhD, David J. Abraham, PhD, Daryll M. Baker, PhD Journal of Vascular Surgery Volume 43, Issue 1, Pages 125-133 (January 2006) DOI: 10.1016/j.jvs.2005.08.042 Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 1 Immunohistochemistry demonstrating CD31-positive microvessels. A, B, The CD31-positive microvessels are visible as dark reddish brown against the background of hematoxylin counterstain. An example of a microvessel is indicated by arrow i, and arrow ii indicates an example of a muscle nuclei. Increased immunopositive microvessels were seen in the CLI group (B) compared with controls (A). Such sections were used for quantitative assessment of microvessels and the microvessel-to-muscle fiber ratio (magnification, ×200). C, Boxplot of microvessel density and (D) microvessel-to-muscle fiber ratio. Boxes represent interquartile range, horizontal lines represent group medians, and the whisker caps represent the 5th and 95th percentiles. Microvessel density was 2.7-fold higher in chronic critical limb ischemia (CLI) muscle biopsy specimens than in controls (P < 0.001, Mann-Whitney U test). The capillary-to-muscle fiber ratio was also significantly higher in CLI compared to controls (P = 0.001, Mann-Whitney U test). Journal of Vascular Surgery 2006 43, 125-133DOI: (10.1016/j.jvs.2005.08.042) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 2 Immunohistochemistry demonstrating hypoxia-inducible factor-1α (HIF-1α) expressions in (A) control and (B) critical limb ischemia (CLI) muscles. There was an increased expression in the CLI muscles, demonstrated by the increased immunoreactivity viewed as dark reddish brown (magnification ×200). The HIF-1α levels were then quantified using Western blotting and enzyme-linked immunoabsorbent assay (ELISA) assay. (C) Boxplot of ELISA assay results of HIF-1α levels in the CLI and control biopsies specimens. Boxes represent interquartile range, horizontal lines represent group medians, and the whisker caps represent the 5th and 95th percentiles. The CLI group has significantly higher HIF-1α expression as represented by optical density (OD) compared with the control group (P < 0.001, Mann-Whitney U test). (D) Western blot of HIF-1α in the control and CLI biopsy specimens. Stronger bands were shown from the CLI biopsies (lanes 5 to 8) at the molecular weight of 120 kD, the molecular weight of HIF-1α. The bands were faintly detectable from biopsies from the control group (lanes 1 to 4). Journal of Vascular Surgery 2006 43, 125-133DOI: (10.1016/j.jvs.2005.08.042) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 3 Serial 6-μm cryosections of critical limb ischemia muscle demonstrate the similar distribution pattern of immunoreactivity between (A) CD31 and (B) HIF-1α. This suggested a strong likelihood of colocalization between the two antigens (magnification ×400). The colocalization was further confirmed by using the double immunofluorescence labelling technique, visualized on a representative single microvessel. (C) HIF-1α was labelled with Texas Red (red). (D) Vascular endothelium expressing CD31 antigen was labelled with fluorescein-5-isothiocyanate (FITC) (green). (E) The colocalization of both HIF-1α and CD31-positive vascular endothelium on the same microvessel is demonstrated by yellow color. Journal of Vascular Surgery 2006 43, 125-133DOI: (10.1016/j.jvs.2005.08.042) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions