Volume 117, Issue 4, Pages (October 1999)

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Volume 117, Issue 4, Pages 770-775 (October 1999) Molecular characterization and functional regulation of a novel rat liver-specific organic anion transporter rlst-1 Masayuki Kakyo, Michiaki Unno, Taro Tokui, Rie Nakagomi, Toshiyuki Nishio, Hajime Iwasashi, Daisuke Nakai, Makoto Seki, Masanori Suzuki, Takeshi Naitoh, Seiki Matsuno, Hiromu Yawo, Takaaki Abe Gastroenterology Volume 117, Issue 4, Pages 770-775 (October 1999) DOI: 10.1016/S0016-5085(99)70333-1 Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 1 (A) Alignment of deduced amino acid sequence of rat rlst-1 and human LST-1. The sequences are aligned with single-letter notation by inserting gaps (−) to achieve the maximum homology. The putative TM segments were assigned. Sequence motifs for potential N-glycosylation sites (triangles) and possible phosphorylation sites (*) are indicated. (B) Hydrophobicity profile was drawn using the algorithm of Kyte and Doolittle15 with a window setting of 15. Sequence motifs for potential N-glycosylation sites (Y) and possible phosphorylation sites (P) are indicated. (C) Phylogenetic relationship between rlst-1, human LST-1, oatp family, and the PG transporter. Branch lengths are drawn to scale. Gastroenterology 1999 117, 770-775DOI: (10.1016/S0016-5085(99)70333-1) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 1 (A) Alignment of deduced amino acid sequence of rat rlst-1 and human LST-1. The sequences are aligned with single-letter notation by inserting gaps (−) to achieve the maximum homology. The putative TM segments were assigned. Sequence motifs for potential N-glycosylation sites (triangles) and possible phosphorylation sites (*) are indicated. (B) Hydrophobicity profile was drawn using the algorithm of Kyte and Doolittle15 with a window setting of 15. Sequence motifs for potential N-glycosylation sites (Y) and possible phosphorylation sites (P) are indicated. (C) Phylogenetic relationship between rlst-1, human LST-1, oatp family, and the PG transporter. Branch lengths are drawn to scale. Gastroenterology 1999 117, 770-775DOI: (10.1016/S0016-5085(99)70333-1) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 1 (A) Alignment of deduced amino acid sequence of rat rlst-1 and human LST-1. The sequences are aligned with single-letter notation by inserting gaps (−) to achieve the maximum homology. The putative TM segments were assigned. Sequence motifs for potential N-glycosylation sites (triangles) and possible phosphorylation sites (*) are indicated. (B) Hydrophobicity profile was drawn using the algorithm of Kyte and Doolittle15 with a window setting of 15. Sequence motifs for potential N-glycosylation sites (Y) and possible phosphorylation sites (P) are indicated. (C) Phylogenetic relationship between rlst-1, human LST-1, oatp family, and the PG transporter. Branch lengths are drawn to scale. Gastroenterology 1999 117, 770-775DOI: (10.1016/S0016-5085(99)70333-1) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 2 Transport of taurocholate in rlst-1–expressing oocytes. The transport rates of [3H]taurocholate for the rlst-1 cRNA-injected oocytes were measured (60 minutes). A representative experiment of 3 is shown. The values indicated are means ± SEM of 5-9 oocyte determinations. Gastroenterology 1999 117, 770-775DOI: (10.1016/S0016-5085(99)70333-1) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 3 Northern blot analysis of rat rlst-1 mRNA. Lane 1, heart; lane 2, brain; lane 3, spleen; lane 4, lung, lane 5, liver; lane 6, skeletal muscle; lane 7, kidney; lane 8, testis. Gastroenterology 1999 117, 770-775DOI: (10.1016/S0016-5085(99)70333-1) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 4 Northern blot analyses for rat rlst-1 and ntcp mRNA in (A) bile duct–ligated rats and (B) CLP rats. The values obtained in sham-operated animals are indicated as the value at day 0. The statistical analysis of the data was performed by analysis of variance, and significance was isolated by Bonferroni's test. Densitometric results are normalized comparing the signals of GAPDH and represented as the mean ± SEM. Gastroenterology 1999 117, 770-775DOI: (10.1016/S0016-5085(99)70333-1) Copyright © 1999 American Gastroenterological Association Terms and Conditions