How does one calculate total magnification on your microscope?

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Presentation transcript:

How does one calculate total magnification on your microscope?

Multiply the magnification of the objective lens by the ocular lens

What is the highest total magnification of your microscope?

What is the highest total magnification of your microscope? 1000x using the oil immersion lens

What is the purpose of using immersion oil?

What is the purpose of using immersion oil? To prevent the refraction of light, which increases the number of light rays which travel up through the objective lens, therefore improving the resolution of the image

Which objective lenses on your scope should you only use the fine adjustment knob?

The oil immersion lens (100X) Which objective lenses on your scope should you only use the fine adjustment knob? The high power lens (45X) The oil immersion lens (100X) These are the two longest lenses on your scope

How should you adjust the light on your microscope?

How should you adjust the light on your microscope? Use the iris diaphragm lever

Describe how to prepare your slide prior to staining

Describe how to prepare your slide prior to staining Slide must be allowed to air dry, then the slide is heat fixed by passing the slide over a bunsen burner two times

What is the purpose of streaking a TSA plate?

What is the purpose of streaking a TSA plate? To isolate bacterial colonies which can be observed for colony morphology To isolate bacterial colonies which could be used to make a pure culture