Hybrid Model Integrating Immunohistochemistry and Expression Profiling for the Classification of Carcinomas of Unknown Primary Site Barbara A. Centeno,

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Presentation transcript:

Hybrid Model Integrating Immunohistochemistry and Expression Profiling for the Classification of Carcinomas of Unknown Primary Site Barbara A. Centeno, Gregory Bloom, Dung-Tsa Chen, Zhihua Chen, Mike Gruidl, Aejaz Nasir, Timothy Y. Yeatman The Journal of Molecular Diagnostics Volume 12, Issue 4, Pages 476-486 (July 2010) DOI: 10.2353/jmoldx.2010.090197 Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Diagram of IHC work flow for the identification of carcinoma from a malignant neoplasm. Initial Cytokeratin IHC separates the neoplasm into positive and negative for CK staining. A second panel of IHC delineates carcinoma from mesothelioma and germ cell tumors. The Journal of Molecular Diagnostics 2010 12, 476-486DOI: (10.2353/jmoldx.2010.090197) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Flow diagram of immunohistochemical staining used to delineate four categories of carcinoma; when available, antibodies used in IHC are shown for primary site of origin identification. Note the absence of primary site of origin antibodies for squamous and urothelial tissues. The Journal of Molecular Diagnostics 2010 12, 476-486DOI: (10.2353/jmoldx.2010.090197) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Flow diagram showing parallel and complementary gene expression classifier and IHC staining used to separate carcinoma into the four major subtypes. Strike thru lines indicate no available IHC. No site of origin classifier was constructed for urothelial as origin site plays no role in treatment decision. The Journal of Molecular Diagnostics 2010 12, 476-486DOI: (10.2353/jmoldx.2010.090197) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions