Dysregulated leukemia inhibitory factor and its receptor regulated signal transducers and activators of transcription 3 pathway: a possible cause for.

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Presentation transcript:

Dysregulated leukemia inhibitory factor and its receptor regulated signal transducers and activators of transcription 3 pathway: a possible cause for repeated implantation failure in women with dormant genital tuberculosis? Elavarasan Subramani, M.Sc., Ejimedo Madogwe, M.Sc., Chaitali Datta Ray, M.D., Subir Kumar Dutta, M.D., Baidyanath Chakravarty, M.D., Vilceu Bordignon, D.V.M., Ph.D., Raj Duggavathi, D.V.M., Ph.D., Koel Chaudhury, Ph.D. Fertility and Sterility Volume 105, Issue 4, Pages 1076-1084.e5 (April 2016) DOI: 10.1016/j.fertnstert.2015.12.015 Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) A representative western blot image of endometrial tissue samples from women with dormant GTB and controls. Protein levels of LIF, LIFR, STAT3, pSTAT3, VEGF, MUC1, MECA-79, CDH1, ITGAV, and ITGB3 were measured. D = dormant GTB; C = controls. (B) Relative quantification of protein expression against β-actin in dormant GTB cases (n = 38) and controls (n = 30) is expressed as mean ± SEM band intensities. *Indicates statistical significance at P<.001 on comparing dormant GTB cases with controls. Fertility and Sterility 2016 105, 1076-1084.e5DOI: (10.1016/j.fertnstert.2015.12.015) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Scanning electron microscope images of pinopodes on the endometrial apical surface of women with dormant GTB and controls during implantation window. (A) Several fully developed pinopodes covering the endometrial surface in controls; (B) poorly developed pinopodes in women with dormant GTB; (C) no/absence of pinopodes in women with dormant GTB; (D) pinopodes score of women with dormant GTB (n = 38) and controls (n = 30) during implantation window. Fertility and Sterility 2016 105, 1076-1084.e5DOI: (10.1016/j.fertnstert.2015.12.015) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Effect of HSP65 on hESC decidualization. (A) Fibroblast-like stromal cells appear in the nondecidual hESCs (ND). Fibroblast-like hESCs decidualized with cAMP+MPA as evidenced by the characteristic feature of polygonal shape (DEC). Conversion of fibroblast-like hESCs into decidual cells are affected by HSP65 treatment (DEC+HSP65). Magnification ×100. (B) mRNA fold change of PRL, IGFBP1, LIF, and LIFR in decidualized hESCs (DEC) and HSP65-treated decidualized hESCs (DEC+HSP65) following adjusted to nondecidualized cells (ND). (C) STAT3 phosphorylation in HSP65-treated decidualized hESCs (DEC+HSP65) compared with untreated decidualized cells (DEC) and nondecidualized cells (ND). Data are represented as the mean ± SEM of three independent experiments. *P<.05 and **P<.001. Fertility and Sterility 2016 105, 1076-1084.e5DOI: (10.1016/j.fertnstert.2015.12.015) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 A flow chart for subject selection. Fertility and Sterility 2016 105, 1076-1084.e5DOI: (10.1016/j.fertnstert.2015.12.015) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Histological section of endometrial tissue from dormant GTB women with (A) only PCR positive, (B) only BACTEC positive, (C) both PCR and BACTEC positive, and (D) control. Magnification ×40. Fertility and Sterility 2016 105, 1076-1084.e5DOI: (10.1016/j.fertnstert.2015.12.015) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 mRNA fold change of ITGAV, ITGB3, MUC1, CDH1, MECA-79, and VEGF in the, decidualized hESCs (DEC) and HSP65 treated decidualized hESCs (DEC+HSP65) following adjusted to nondecidualized cells (ND). Fertility and Sterility 2016 105, 1076-1084.e5DOI: (10.1016/j.fertnstert.2015.12.015) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions