Weiguo Chen, MD, PhD, Yasuhiro Tabata, MD, PhD, Aaron M

Slides:

Advertisements

Similar presentations

Murine leptin deficiency alters Kupffer cell production of cytokines that regulate the innate immune system Zhiping Li, Huizhi Lin, Shiqi Yang, Anna.

Advertisements

Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components Stephen R. Reeves, MD, PhD, Tessa.

Surface availability of beta-glucans is critical determinant of host immune response to Cladosporium cladosporioides Rachael A. Mintz-Cole, PhD, Eric.

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.

Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma Kyung S. Lee, MS, Sun M. Jin,

IL-4 induces IL-13–independent allergic airway inflammation

Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M

Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,

Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications Elena Goleva,

Umasundari Sivaprasad, PhD, David J. Askew, PhD, Mark B

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice Azzeddine Dakhama, PhD, Young-Mok Lee,

Proviral integration site for Moloney murine leukemia virus 1, but not phosphatidylinositol-3 kinase, is essential in the antiapoptotic signaling cascade.

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation Yuki Nakamura,

Distinct TLR-mediated pathways regulate house dust mite–induced allergic disease in the upper and lower airways Ji-Hwan Ryu, PhD, Jung-Yeon Yoo, BS,

Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring Phoebe K. Richgels, MS, Amnah.

Allergen-dependent solubilization of IL-13 receptor α2 reveals a novel mechanism to regulate allergy Michael O. Daines, MD, Weiguo Chen, PhD, Yasuhiro.

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,

IL-33 induces innate lymphoid cell–mediated airway inflammation by activating mammalian target of rapamycin Robert J. Salmond, PhD, Ananda S. Mirchandani,

Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation Si-Yen Liu, PhD, Wei-Zhi Wang,

Β-Glucan exacerbates allergic asthma independent of fungal sensitization and promotes steroid-resistant TH2/TH17 responses Zhonghua Zhang, MD, Jocelyn.

Is 9 more than 2 also in allergic airway inflammation?

Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.

Dendritic cells and alveolar macrophages mediate IL-13–induced airway inflammation and chemokine production Margaret Crapster-Pregont, BS, Janice Yeo,

Surfactant protein D inhibits TNF-α production by macrophages and dendritic cells in mice László Hortobágyi, MS, Sonja Kierstein, PhD, Kateryna Krytska,

Dawn C. Newcomb, PhD, Madison G

Ozone exposure induces respiratory barrier biphasic injury and inflammation controlled by IL-33 Chloé Michaudel, PhD, Claire Mackowiak, MSc, Isabelle.

Chang Xiao, MD, PhD, Jocelyn M

Role of TWIK-related potassium channel-1 in chronic rhinosinusitis

CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling Jennifer Kearley, PhD, Douglas S. Robinson,

IL-13 desensitizes β2-adrenergic receptors in human airway epithelial cells through a 15-lipoxygenase/G protein receptor kinase 2 mechanism Giusy D.

Mechanisms of tissue inhibitor of metalloproteinase 1 augmentation by IL-13 on TGF- β1–stimulated primary human fibroblasts Xiuxia Zhou, PhD, Haizhen.

FcɛRI cross-linking and IL-3 protect human basophils from intrinsic apoptotic stress Lionel Rohner, MSc, Ramona Reinhart, PhD, Björn Hagmann, PhD, Andrea.

Surface availability of beta-glucans is critical determinant of host immune response to Cladosporium cladosporioides Rachael A. Mintz-Cole, PhD, Eric.

Corticosteroid-resistant asthma is associated with classical antimicrobial activation of airway macrophages Elena Goleva, PhD, Pia J. Hauk, MD, Clifton.

IL-2–inducible T-cell kinase modulates TH2-mediated allergic airway inflammation by suppressing IFN-γ in naive CD4+ T cells Arun K. Kannan, MS, Nisebita.

Ozone exposure induces respiratory barrier biphasic injury and inflammation controlled by IL-33 Chloé Michaudel, PhD, Claire Mackowiak, MSc, Isabelle.

Antiviral activity of human β-defensin 3 against vaccinia virus

Mast cell–derived plasminogen activator inhibitor type 1 promotes airway inflammation and remodeling in a murine model of asthma Ara Jo, PhD, Sun H.

Cold temperature induces mucin hypersecretion from normal human bronchial epithelial cells in vitro through a transient receptor potential melastatin.

Allergen-induced IL-6 trans-signaling activates γδ T cells to promote type 2 and type 17 airway inflammation Md Ashik Ullah, MPharm, Joana A. Revez,

Does reduced zona pellucida binding protein 2 (ZPBP2) expression on chromosome 17q21 protect against asthma? Marina Miller, MD, PhD, Christine Vuong,

TNF-α induces the late-phase airway hyperresponsiveness and airway inflammation through cytosolic phospholipase A2 activation Il-Whan Choi, PhD, Sun-Kim,

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

Altered gene expression profiles in nasal respiratory epithelium reflect stable versus acute childhood asthma Jesus R. Guajardo, MD, MHPE, Kathleen W.

The Editors' Choice Journal of Allergy and Clinical Immunology

IL-17A enhances IL-13 activity by enhancing IL-13–induced signal transducer and activator of transcription 6 activation Sara L. Hall, MS, Theresa Baker,

Update on the role of prostaglandins in allergic lung inflammation: Separating friends from foes, harder than you might think Martin L. Moore, PhD, R.

Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD

Weiguo Chen, PhD, Gurjit K. Khurana Hershey, MD, PhD

Novel allergic asthma model demonstrates ST2-dependent dendritic cell targeting by cypress pollen Lucia Gabriele, BS, Giovanna Schiavoni, BS, Fabrizio.

Regulation of ectodomain shedding of ADAM33 in vitro and in vivo

Xin-Zi Tang, PhD, James B. Jung, BS, Christopher D.C. Allen, PhD

Autophagy: Nobel Prize 2016 and allergy and asthma research

Stephanie A. Shore, PhD, Raya D

Effect of IL-13 receptor α2 levels on the biological activity of IL-13 variant R110Q Allison-Lynn Andrews, PhD, Fabio Bucchieri, MD, Kazuhiko Arima, MD,

12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels Amanda R. Hajek, BS, Alexa R. Lindley,

Duy Pham, PhD, Sarita Sehra, PhD, Xin Sun, PhD, Mark H. Kaplan, PhD

Unique and overlapping gene expression patterns driven by IL-4 and IL-13 in the mouse lung Christina C. Lewis, PhD, Bruce Aronow, PhD, John Hutton, MD,

Matrix metalloproteinase inhibitor regulates inflammatory cell migration by reducing ICAM-1 and VCAM-1 expression in a murine model of toluene diisocyanate-induced.

Douglas A. Kuperman, PhD, Christina C. Lewis, PhD, Prescott G

Eric B. Brandt, PhD, Melissa K. Mingler, MS, Michelle D

Macrolide antibiotics and asthma treatment

Asthma: The past, future, environment, and costs

Phosphoinositide 3-kinase-δ inhibitor reduces vascular permeability in a murine model of asthma Kyung Sun Lee, PhD, Seoung Ju Park, MD, So Ri Kim, MD,

Junqing Cui, PhD, Stephen Pazdziorko, Joy S

Natural history of cow’s milk allergy

TNF can contribute to multiple features of ovalbumin-induced allergic inflammation of the airways in mice Susumu Nakae, PhD, Carolina Lunderius, PhD,

IL-2–inducible T-cell kinase modulates TH2-mediated allergic airway inflammation by suppressing IFN-γ in naive CD4+ T cells Arun K. Kannan, MS, Nisebita.

Presentation transcript:

Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor α2 in vivo Weiguo Chen, MD, PhD, Yasuhiro Tabata, MD, PhD, Aaron M. Gibson, BS, Michael O. Daines, MD, Manoj R. Warrier, MD, Marsha Wills-Karp, PhD, Gurjit K. Khurana Hershey, MD, PhD Journal of Allergy and Clinical Immunology Volume 122, Issue 3, Pages 625-632 (September 2008) DOI: 10.1016/j.jaci.2008.06.022 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Levels of soluble IL-13Rα2 in serum and BALF and the expression of MMPs in lungs from PBS- or HDM-treated FVB/N mice. A and B, Soluble IL-13Rα2 in serum and BALF. Data were shown as means ± SD (n = 4-5). ∗P < .05. The result is representative of 3 experiments. C, RNase protection assay of the RNA from the lungs of PBS- or HDM-treated mice (n = 4). TIMP, Tissue inhibitor of metalloproteinases; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Journal of Allergy and Clinical Immunology 2008 122, 625-632DOI: (10.1016/j.jaci.2008.06.022) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Acellular cleavage of GST–IL-13Rα2–FLAG fusion protein by MMPs. A, Cleavage of GST–mIL-13Rα2–FLAG. B and C, Cleavage of GST or GST–mIL-13Rα2–FLAG by MMP-8. D and E, Cleavage of GST–mIL-13Rα2–FLAG or human collagen type I by MMP-8 in the presence or absence of an MMP-8 inhibitor or mock MMP-8 inhibitor control (5, 50, or 500 nmol/L). F, Cleavage of GST–hIL-13Rα2–FLAG. Journal of Allergy and Clinical Immunology 2008 122, 625-632DOI: (10.1016/j.jaci.2008.06.022) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Measurement of soluble IL-13Rα2 in the culture medium and on the cell surface after MMP-8 treatment. Soluble IL-13Rα2 levels are shown in panels A and B. IL-13Rα2 surface expression is shown as mean channel fluorescence (MCF) in panels C and D. Data are shown as means ± SD (n = 3-5). ∗P < .05. The result is representative of 3 experiments. Journal of Allergy and Clinical Immunology 2008 122, 625-632DOI: (10.1016/j.jaci.2008.06.022) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 IL-13 binding of soluble IL-13Rα2 in culture medium from MMP-8–treated cells. The result is representative of 3 experiments. A, MMP-8 treatment of untransfected or mIL-13Rα2–transfected WEHI cells. The data are shown as means ± SD (n = 5). ∗P < .05. B, Western blot analysis of MMP-8 treatment of murine GST–IL-13Rα2–FLAG fusion protein for the indicated time (0.5-24 hours). U, Untreated. Journal of Allergy and Clinical Immunology 2008 122, 625-632DOI: (10.1016/j.jaci.2008.06.022) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 AHR, airway inflammation, BALF soluble IL-13Rα2 and its IL-13 binding activity, and BALF IL-13 of PBS- or HDM-treated wild-type and MMP-8–deficient mice. A, APTI (n = 6-8). B, BAL cell counting (n = 6-8). C, Soluble IL-13Rα2 (n = 6-8). D, IL-13 binding activity of soluble IL-13Rα2 (n = 12-17, pooled from 2 experiments). E, Free and IL-13Rα2–bound IL-13 (n = 5). WT, Wild-type mice; KO, MMP-8–deficient mice. The data are shown as means ± SD. ∗P < .05. Journal of Allergy and Clinical Immunology 2008 122, 625-632DOI: (10.1016/j.jaci.2008.06.022) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions