Almost all of molecular biology techniques are based on this paper

Figure 1 Slow cooling was better 2) More DNA more transformation activity

Activity correlates to change in absorption of cooled DNA not warm DNA Figure 2 DNA absorption at heated temp DNA absorption after cooling Activity correlates to change in absorption of cooled DNA not warm DNA

Figure 3

Figure 4 Fast cooling? 1ug/ml to 20ug/ml

Figure 5 Salt dependent Annealing by Repressing the repulsive Effect of phosphates By shielding them.

Figure 6 Non-homologous DNA makes it Harder for homologous ssDNA to find each other

TCA TC/T TGC TTC ACA ATG GTA/G CTC/T TTC/T CCA/G GAT TC S S C F T M V L F P D TC GAA TCC/T GGA/G AAA/G AGT/C ACC ATT GTG AAG CAA/G ATG E S G K S T I V K Q M

E S G K S T I V K Q M