A Promiscuous Survivin-Derived T-Cell Epitope Restricted to the HLA-A3 Super-Type Alleles Niels Junker, Shamaila Munir, Pia Kvistborg, Per thor Straten,

Slides:

Advertisements

Similar presentations

Volume 17, Issue 9, Pages (September 2015)

Advertisements

Volume 14, Issue 1, Pages (January 2012)

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

Volume 19, Issue 12, Pages (December 2011)

Depletion of Alloreactive Donor T Lymphocytes by CD95-Mediated Activation-Induced Cell Death Retains Antileukemic, Antiviral, and Immunoregulatory T Cell.

In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells Martine A.

Indoleamine 2,3-dioxygenase specific, cytotoxic T cells as immune regulators by Rikke Bæk Sørensen, Sine Reker Hadrup, Inge Marie Svane, Mads Christian.

Peptide Immunization Indicates that CD8+ T Cells are the Dominant Effector Cells in Trinitrophenyl-Specific Contact Hypersensitivity Stefan Martin, Michael.

Antigen-Specific CD8 T Cells Can Eliminate Antigen-Bearing Keratinocytes with Clonogenic Potential via an IFN-γ-Dependent Mechanism Rachel L. De Kluyver,

UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin Amine Achachi,

ALK as a novel lymphoma-associated tumor antigen: identification of 2 HLA-A2.1–restricted CD8+ T-cell epitopes by Lorena Passoni, Antonio Scardino, Carla.

Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis

Natural Killer and Dendritic Cell Contact in Lesional Atopic Dermatitis Skin –Malassezia- Influenced Cell Interaction Eva Buentke, Lena C. Heffler, Annika.

Ex vivo induction of multiple myeloma–specific cytotoxic T lymphocytes

CD8+ cytotoxic T lymphocytes isolated from allogeneic healthy donors recognize HLA class Ia/Ib–associated renal carcinoma antigens with ubiquitous or restricted.

Volume 143, Issue 4, Pages e9 (October 2012)

Volume 137, Issue 2, Pages (August 2009)

Mature dendritic cells pulsed with freeze–thaw cell lysates define an effective in vitro vaccine designed to elicit EBV-specific CD4+ and CD8+ T lymphocyte.

HLA-A*0201+ Plasmacytoid Dendritic Cells Provide a Cell-Based Immunotherapy for Melanoma Patients Caroline Aspord, Marie-Therese Leccia, Dimitri Salameire,

Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells Zia U.A. Mollah, Setsuya Aiba,

Methods to Improve Adoptive T-Cell Therapy for Melanoma: IFN-γ Enhances Anticancer Responses of Cell Products for Infusion Marco Donia, Morten Hansen,

Efalizumab Therapy for Atopic Dermatitis Causes Marked Increases in Circulating Effector Memory CD4+ T Cells That Express Cutaneous Lymphocyte Antigen

Volume 17, Issue 9, Pages (September 2009)

Cytomegalovirus-Specific Cytotoxic T Lymphocytes Can Be Efficiently Expanded from Granulocyte Colony-Stimulating Factor–Mobilized Hemopoietic Progenitor.

Mutated regions of nucleophosmin 1 elicit both CD4+ and CD8+ T-cell responses in patients with acute myeloid leukemia by Jochen Greiner, Yoko Ono, Susanne.

Volume 7, Issue 1, Pages (July 1997)

Induction of chronic lymphocytic leukemia (CLL)–specific CD4- and CD8-mediated T-cell responses using RNA-transfected dendritic cells by Martin R. Müller,

Melanoma Cells Control Antimelanoma CTL Responses via Interaction between TIGIT and CD155 in the Effector Phase Takashi Inozume, Tomonori Yaguchi, Junpei.

Volume 134, Issue 4, Pages (April 2008)

HLA-A*0201+ Plasmacytoid Dendritic Cells Provide a Cell-Based Immunotherapy for Melanoma Patients Caroline Aspord, Marie-Therese Leccia, Dimitri Salameire,

Christian Möbs, Thomas Schmidt Journal of Investigative Dermatology

Circulating Natural Killer Lymphocytes Are Potential Cytotoxic Effectors Against Autologous Malignant Cells in Sezary Syndrome Patients Jean-David Bouaziz,

Positive Atopy Patch Test Reaction to Malassezia furfur in Atopic Dermatitis Correlates with a T Helper 2-like Peripheral Blood Mononuclear Cells Response

Induction of T-Cell Responses against Cutaneous T-Cell Lymphomas Ex Vivo by Autologous Dendritic Cells Transfected with Amplified Tumor mRNA Xiao Ni,

Skin CD4+ T Cells Produce Interferon-γIn Vitro in Response to Streptococcal Antigens in Chronic Plaque Psoriasis Dean W. Brown, Barbara S. Baker, Jean-Marc.

Volume 127, Issue 3, Pages (September 2004)

Drug Specific Cytotoxic T-Cells in the Skin Lesions of a Patient with Toxic Epidermal Necrolysis Amal Nassif, Armand Bensussan, Nicolas Bachot, Martine.

A comparison of gene transfer and antigen-loaded dendritic cells for the generation of CD4+ and CD8+ cytomegalovirus-specific T cells in HLA-A2+ and HLA-A2−

T Cells and T Cell-Derived Cytokines as Pathogenic Factors in the Nonallergic Form of Atopic Dermatitis Cezmi A. Akdis, Mübeccel Akdis, Dagmar Simon,

Measurement of CD4+ and CD8+ T-Lymphocyte Cytokine Secretion and Gene Expression Changes in p-Phenylenediamine Allergic Patients and Tolerant Individuals

Clinical Snippets Journal of Investigative Dermatology

Minutes of the Board of Directors Meeting

Jürgen C. Becker, David Schrama Journal of Investigative Dermatology

Volume 14, Issue 2, Pages (February 2001)

Volume 13, Issue 2, Pages (February 2006)

Melanoma Inhibitor of Apoptosis Protein (ML-IAP) Specific Cytotoxic T Lymphocytes Cross-React with an Epitope from the Auto-Antigen SS56 Rikke Bæk Sørensen,

Volume 18, Issue 9, Pages (September 2010)

PPARδ Is a Type 1 IFN Target Gene and Inhibits Apoptosis in T Cells

Journal of Investigative Dermatology

MHC-Dependent and -Independent Activation of Human Nickel-Specific CD8+ Cytotoxic T Cells from Allergic Donors1 Corinne Moulon, Doris Wild, Hans Ulrich.

Molecular Features of Penicillin Allergy

Society for Investigative Dermatology 2010 Meeting Minutes

Tomoo Ohashi Journal of Investigative Dermatology

Mads Hald Andersen, Jürgen C. Becker, Per thor Straten

Cord Blood Nucleated Cells Induce Delayed T Cell Alloreactivity

Double-Stranded RNA-Exposed Human Keratinocytes Promote Th1 Responses by Inducing a Type-1 Polarized Phenotype in Dendritic Cells: Role of Keratinocyte-Derived.

Volume 8, Issue 2, Pages (August 2003)

Mads Hald Andersen, Sine Reker, Jürgen C. Becker, Per thor Straten

Epicutaneous Application of CpG Oligodeoxynucleotides with Peptide or Protein Antigen Promotes the Generation of CTL Sandra K. Klimuk, Hossain M. Najar,

Volume 24, Issue 6, Pages (June 2016)

Melanoma Cells Control Antimelanoma CTL Responses via Interaction between TIGIT and CD155 in the Effector Phase Takashi Inozume, Tomonori Yaguchi, Junpei.

Marta E. Polak, Louise Newell, Vadim Y

Interleukin-17A Promotes IgE Production in Human B Cells

Bcl-2 and bcl-xL Antisense Oligonucleotides Induce Apoptosis in Melanoma Cells of Different Clinical Stages Robert A. Olie, Christoph Hafner, Renzo Küttel,

Specific recognition of HOM-MEL-40/SSX2-derived p pulsed targets by p stimulated T-cells from patients with HOM-MEL-40/SSX2 positive breast.

Innate Immunity Stimulates Permeability Barrier Homeostasis

Cytotoxicity of p5399–107-induced CTL cells.

HLA-A*0201 restriction of SSX2-derived p stimulated CD8+ T-cells.

Cytotoxic activity of CD8+ T-cells stimulated with SSX2-derived p

Design and operation of p.DOM-epitope vaccines.

Presentation transcript:

A Promiscuous Survivin-Derived T-Cell Epitope Restricted to the HLA-A3 Super-Type Alleles Niels Junker, Shamaila Munir, Pia Kvistborg, Per thor Straten, Inge Marie Svane, Mads Hald Andersen Journal of Investigative Dermatology Volume 132, Issue 8, Pages 2115-2118 (August 2012) DOI: 10.1038/jid.2012.109 Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 IFN-γ release. Analysis of IL-2–expanded tumor-infiltrating lymphocyte (TIL) functionality was initiated by enzyme-linked immunospot (Elispot) IFN-γ quantification (Andersen et al., 2001). Tests were conducted in triplicates preferably and in duplicates depending on cell availability. (a) Identification of SUR53-62–specific TILs among cancer patients measured by IFN-γ release in Elispot. Values of spot-forming cells (SFCs) are presented as peptide-stimulated TILs after subtraction of unstimulated TILs serving as background values. Significant SUR53-62 responses (black circles) were identified in seven of eight patients, ranging from 49 to 187 SFCs. Examples of nonsignificant responses (gray circles) from TILs derived from the same fragments are shown as controls. TILs contained mainly T cells with an equal distribution of CD8+ T cells among nonsignificant (range=46–96%, mean=78.9%, SD=19%) and significant (range=15–98%, mean=79.8, SD=28.7) SUR53-62–responding cultures. (b) Representative wells from a highlighted culture (patient MM 1), comparing low-background counts in unstimulated TILs (-) and three known HLA-A3–restricted tumor-associated antigens (TAAs), which did not show responses in relation to a significant SUR53-62 response. The TAAs are (1) Bcl-X(L) 165–173 (RIAAWMATY) (Andersen et al., 2005); (2) Rho1 (RAGLQVRKNK); and (3) Rho1L2 (RLGLQVRKNK) (Wenandy et al., 2008). Journal of Investigative Dermatology 2012 132, 2115-2118DOI: (10.1038/jid.2012.109) Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Lytic capacity was analyzed by FACS and Cr-release assay. Tests were conducted in triplicates preferably and in duplicates depending on cell availability. (a) Identification of CD107a-positive T-cell populations among CD8+ tumor-infiltrating lymphocytes (TILs) in two separate cultures (M4 and M6) upon SUR53-62 stimulation in a HLA-A3+/A11+ and a HLA-A3+ patient, respectively. Unstimulated TILs and the irrelevant peptide HIV C31 show equal low-background staining. Stimulants were added directly into wells with TILs. (b) Further assessment by Cr-release assay showed specific killing of SUR53-62–pulsed T2 cells restricted to either HLA-A3 or HLA-A11. TILs from M1 (97% CD8+) show T2-A11–restricted killing and no lysis of T2-A3 cells presenting the SUR53-62 peptide. TILs from M4 (15% CD8+) from the same patient induce specific killing directed against the SUR53-62–pulsed T2-A3+ cells. Effector:target (E:T) ratios are CD8 corrected. (c) Identification of the cytotoxic capacity of TILs (92% CD8+) from a HLA-A3+ patient, against an autologous tumor cell line and T2-A3 cells presenting SUR53-62 (P=0.01). The complete alignment of lytic activity suggests that this particular survivin-derived epitope, presented by HLA-A3, has an impact on the reinduced immune-mediated tumor cell destruction. Journal of Investigative Dermatology 2012 132, 2115-2118DOI: (10.1038/jid.2012.109) Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions