MEIOSIS … making gametes….

Gamete = sex cell (egg and sperm) Each gamete has 1 set of 23 chromosomes When they come together, the resulting cell has 46 chromosomes Somatic cell = body cells (all cells except eggs and sperm) 23 23

Cells with 1 set of chromosomes (gametes) are said to be HAPLOID (n) Each of your somatic (body) cells has 2 sets of chromosomes – one from mom, one from dad. Any cell that has 2 complete sets of chromosomes is said to be DIPLOID (2n) Cells with 1 set of chromosomes (gametes) are said to be HAPLOID (n) When 2 gametes come together in fertilization, they make a zygote. The zygote is diploid and has 46 chromosomes.

Practice! If a goldfish’s diploid number is 42, what is its haploid number? If a turtle’s sperm cell has 50 chromosomes, how many chromosomes would a turtle’s skin cell contain? (Sperm cells are gametes, which are haploid. Skin cells are somatic cells, which are diploid.)

What type of cells produce eggs and sperm? Ovarian and testicular cells!

Why do meiosis? Why do ovary and testes cells go through meiosis? Meiosis is “reduction division”. During cell division, it reduces the chromosome number in the gametes by half. From diploid (2n) to haploid (n). Why do meiosis?

Meiosis is… The process of nuclear division that reduces the number of chromosomes by half Somatic cell  Gamete Making the cells that make babies! (egg and sperm)

Homologous Chromosomes (pairs) In each somatic (body) cell you have 23 homologous pairs Similarities: length, centromere location, coded traits, banding pattern Differences: one chromosome from each parent

Homologous Chromosomes have the same banding pattern

Karyotype - picture of all chromosomes in the nucleus of 1 cell Used to determine: If there is an abnormality in number or structure of the chromosomes (e.g. Down’s syndrome) 2. Gender

Diploid Testicular cell Diploid ovarian cell Diploid Testicular cell How you got here: 2n 2n egg (n) sperm (n) fertilization zygote MEIOSIS MEIOSIS MITOSIS You

Products of MITOSIS: Products of MEIOSIS: 2 identical diploid (2n) cells Products of MEIOSIS: 4 unique haploid (n) cells   2n 2n + 2n 2n n + n + n + n

In streptomycin fungus n = 11 In streptomycin fungus n = 11. What is the diploid number for this species? If n = 15 for goldfish, how many chromosomes would be in a fin cell? If 2n = 108 for black spruce trees, what is the haploid number? 4. During MITOSIS, the chromosomes A. are located at the cell equator during prophase B. are located at the cell equator during telophase C. move toward the poles of the cell during anaphase D. move toward the poles of the cell during metaphase

Meiosis I Meiosis II

MEIOSIS I 1. INTERPHASE I: Period of cell growth and preparation for division (G1, S, G2) PROPHASE I: Tetrads appear Crossing Over (synapsis) occurs - Before Interphase Homologous chromosome pair After interphase tetrad Crossing over Recombinant chromatids

METAPHASE I: Tetrads (2 homologous chromosomes) line up on metaphase plate and lock on to spindle fibers  ANAPHASE I: Spindle fibers pull tetrads (chromosome pairs) apart. Independent assortment occurs.  

TELOPHASE I: nuclear membranes form around the two new nuclei CYTOKINESIS I: cell splits in half. Result = 2 haploid cells

MEIOSIS II

Special Interphase between the two divisions: NO replication!!! - nothing changes with the chromosomes between telophase I and prophase II

Prophase II: Sister chromatids condense from chromatin. Metaphase II: Sister chromatids line up on equator Anaphase II: Sister chromatids are pulled apart. Telophase II: Nuclear membranes form.

Total product is 4 haploid (n), genetically DIFFERENT gametes Cytokinesis II: cytoplasm divides Total product is 4 haploid (n), genetically DIFFERENT gametes

Why Meiosis is important: It creates Genetic Recombination (variety) Resulting from: crossing over independent assortment Crossing Over: Exchange of genetic material between homologous chromosomes during prophase I of Meiosis

Crossing Over Exchange of genetic material between homologous chromosomes during Prophase I Notice that these chromosomes have been duplicated. When does DNA replication happen?

Independent Assortment Creating a random mix of genes in each gamete, based on how tetrads line up at Metaphase I

https://www.youtube.com/watch?v=16enC385R0w http://www.cellsalive.com/meiosis.htm https://www.youtube.com/watch?v=iCL6d0OwKt8

Meiosis vs. Mitosis (Now, fill in your Venn Diagram!) Used for Sexual Reproduction Produces Gametes (Egg & Sperm) Have 23 chromosomes 2 sets of divisions Begins with a diploid cell Homologous chromosomes pair up Result: 4 genetically different HAPLOID SEX cells Used for Growth and Asexual Reproduction Produces body cells Have 46 chromosomes Somatic cells One round of division Begins with diploid cells No crossing over Homologous chromosomes don’t pair up Results in 2 genetically identical DIPLOID SOMATIC cells (Now, fill in your Venn Diagram!)

Chromosome number questions… If a horse retina (eye) cell has 36 chromosomes, how many does a horse egg cell have? If n = 4 for fruit flies, how many chromosomes does a wing cell have? 3. If the 2n number of a pine tree is 86, how many chromosomes would be found in a pine ovum (egg)?

Non-disjunction Non-separation of chromosomes resulting in diploid or empty gametes When it happens in Meiosis I: All 4 gametes affected When it happens in Meiosis II: Half of gametes affected

Conditions caused by non-disjunction Down’s syndrome = Trisomy 21 - 3 copies of chromosomes 21 equaling a total of 47 (2n + 1) chromosomes. chance of occurring in oogenesis increases with maternal age Turner’s syndrome = Monosomy X - has only one X chromosomes equaling a total of 45 (2n-1) chromosomes in her body cells.

Down’s syndrome – Trisomy 21 A simple check for the presence of a bone in the nose could more accurately test unborn babies for Down's syndrome, scientists say. Researchers say combining the nose test with existing screening methods (amniocentesis) could lead to a five-fold reduction in the number of miscarriages linked to an invasive procedure used to confirm Down's syndrome.

Turner’s syndrome X -Caused by missing or incomplete X chromosome -Genes affected are involved with growth and sexual development -May fail to start puberty -Shorter than normal -Treatment = Hormone Replacement Therapy

Klinefelter’s syndrome XXY Normal boy who develops some female secondary sex characteristics at puberty. -slightly lower IQ, infertile, delayed motor, speech, maturation -treated with testosterone