Supplemental Figure 1 A B C.

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Supplemental Figure 1 A B C

Supplemental Figure 2 57 kDa 70 kDa c-myc β-actin 42 kDa RWPE-1 DU-145 PC-3 PC-3-ML AR-CaP-E AR-CaP-M c-myc β-actin 42 kDa

Supplemental Figure 3 A B

Supplemental Figure 4 pPEG-Luc pAEG-Luc 24h 48h 24h 48h D V L R PC3-ML PCa-1 PCa-2 PCa-3 PC3-ML PCa-1 PCa-2 PCa-3 PCa-4 Control Ctrl-1 Ctrl-2 Ctrl-3 Control Ctrl-1 Ctrl-2 Ctrl-3

Supplemental Figure 5 (Normalized ng Luc pDNA)/(100ng gDNA) AEG-Prom treated PCa Lungs Tumor PEG-Prom treated NS

Supplemental Figure 6 T A B C D E Gross H&E IHC(Anti-Luc) Pathology In-vivo BLI Ex-vivo BLI Gross H&E IHC(Anti-Luc) Pathology A B C D T E

Supplemental Figure 7 A B PC3-ML Control PCa-1 Ctrl-1 PCa-2 Ctrl-2

Supplemental Figure 8 L2 B A L2 L1 L3, L4 L5 C