Gram stain Marion Schoof.

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Presentation transcript:

Gram stain Marion Schoof

Background information Gram stain important Method for differencaiation and classification of bacteria Bacteria devided into two classes: Gram-positive Gram-negative

Staining: solution Time Process Cristal Violet, alcoholic 00:01:30 Coloring Aqua nondest 00:00:30 Elution Lugol´s Solution 00:03:00 00:00:20 Decoloring-solution of Gram 00:00:10 Decoloring Safranin-Solution 00:01:00 Coloring (Counterstain)

Afterwards dehydration in: Ethanol 96% (2 x 2min) Isopropanol (1 x 2min) Xylel (2 x 5 min)

Automatic staining and manual staining perfomed according to the same schedule Elution under running water

Images manual staining: