Quantitative Phase Imaging Device

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Presentation transcript:

Quantitative Phase Imaging Device By: Kyle Riley

Quantitative Phase Imaging (QPI) QPI is an emerging field aimed at studying weakly scattering and absorbing specimens. The measured image in QPI is a map of pathlength shifts associated with the specimen.

Blood Sample Prep Diluted in PBS solution with 0.1% albumin to a concentration of 0.2% whole blood in solution Sample chamber created The cells are allowed to settle for 5 minutes prior to measurement.

White Light Diffractive Phase Microscopy wDPM Developed an integrated hardware-software system that can measure detailed information from thousands of cells in a blood film in real-time. Retrieve the optical pathlength map associated with the blood film.

Measurements Projected area Phase map conversion to height map Once the height information is retrieved, the volume of each cell is calculated by integrating the height map over the projected area The surface area of individual cells is determined using Monge parameterization, where the contribution of each pixel element, dA, can be calculated From the surface area and volume, we calculate parameters such as sphericity ( ) and minimum cylindrical diameter (MCD).

Measurements

The motive behind the quantitative phase imaging (QPI) device is to allow economically challenged countries and rural areas to gain access to modern blood testing. This QPI device offers accurate blood screens with little human interaction, allowing for an increase in blood screening due to a decrease in human labor going into each test.

Resoucres [1] Pham et al. (2013) Real Time Blood Testing Using Quantitative Phase Imaging. PLoS ONE 8(2): e55676. doi:10.1371/journal.pone.0055676 [2] Wood Et Al. "Problems With Blood Transfusions." Trauma and Orthopedic Surgery In Clinical Practice 4 (2009): 131-34. Springer. Web. [3] For People of African, Mediterranean, or Southeast Asian Heritage: Important Information About Diabetes Blood Tests. Bethesda, MD: U.S. Dept. of Health and Human Services, NIDDK, National Institutes of Health, 2007. Internet resource. AQ