Koshi high school Erina Mizutani Shiori Endo Study of plant dyes Koshi high school Erina Mizutani Shiori Endo

antibacterial properties Dyeing cloth with foods which have antibacterial properties. Does this cloth also have antibacterial properties? food

Test 1 Purpose

onion

umeboshi

azuki beans

grape juice

coffee

honey

Karashi (mustard)

wasabi

Do you know umeboshi？ traditional Japanese food very sour! with rice!

Method 5%Alum

onion grape

PDA Culture Plate P・・・potato D・・・dextrose A・・・agar

Result

onion AP × Color ×

umeboshi AP ○ Color ○

AP ○ Color ○ azuki beans

grape AP × Color ×

coffee AP × Color ×

honey AP × Color ×

karashi AP × Color ×

wasabi AP × Color ×

antibacterial properties color onion × umeboshi ○ azuki beans grape coffee honey karashi wasabi

Consideration We think the antibacterial properties are involved in the color change.

Test 2 Purpose We　extracted Umeboshi’s color.

Method ①Mash Umeboshi into a paste. ②Put the samples in ethanol, methanol, hexane, and chloroform. ③Shake them. Umeboshi paste

Result Solvent ↓ red Solvent ↓ yellow methanol ethanol Solvent ↓ No change 　　　 chloroform 　　　　hexane

Consideration ・Ethanol has a high polarity. ・We can analyze the extracted color with chromatography.

Summary ①The cloth dyed with Umeboshi, Azuki beans, and Wasabi have antibacterial properties. ②We think the antibacterial properties are involved in the color change. ③Ethanol is good at extracting Umeboshi’s color.

Issues ・We have to analyze umeboshi’s color, making sure to single out that color, and reveal a relationship between its color and its antibacterial properties. ・We have to extract and analyze the color from “red shiso”, and reveal that it also has antibacterial properties color

We think・・・ The cloths don’t have antibacterial properties. The cloths have antibacterial properties. germ detector germ detector antibacterial properties

Bibliography http://jccu.coop/kodomo_lamd/freestudy/kusakizome/kusa_02.html 「草木染めに挑戦しよう！」 http://slism.jp/calorie/106095/ 「カロリーSlism」 http://www.ciaojapan.org/manual_tanekin_kinsibaiyo.html 「キノコ栽培サイト」

Thank you very much for your kind attention!!!