Large-Scale Production of Recombinant Proteins Lecture 6

Slides:



Advertisements
Similar presentations
Bioreactor Analysis and Operation Chapter 9&10 (textbook)
Advertisements

Microbial Growth Kinetics
Fermentation Technology
Pure culture techniques laboratory (for bacterial cultures) OR Common sense laboratory! Jeet Kalia
Drug Delivery & Tissue Engineering Laboratory
Oxygen transfer in bioreactors
Chapter 3: Bioreactor Design
Fermentation products (Biomass). Principle Fermentation: it is a metabolic process that converts complex organic compounds (ex: sugars) into simpler ones.
General Microbiology (Micr300) Lecture 4 Nutrition and Growth (Text Chapters: ; 6.1; ; )
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology.
Production Of Enzymes By Fermentation Method
Biotechnology practical course Second Year Clinical.
Introduction to Fermentation
TYPES TYPES OF FERMENTER
Chapter 3: Types of BioreactorS
F215 control, genomes and environment
Choosing the Cultivation Method
Pretreatment Application of Ligninolytic Enzymes Faculty Sponsor: Dr. Christine Kelly School of CBEE Group Members: Uranbileg Daalkhaijav, Faraz Ebrahimi,
Microfluidics Technology Fair, October 3, 2006 Parallel Integrated Bioreactor Arrays for Bioprocess Development Harry Lee, Paolo Boccazzi, Rajeev Ram,
What is a Fermenter? Vessel or tank in which whole cells or cell-free enzymes transform raw materials into biochemical products and/or less undesirable.
Microbial Biotechnology Commercial Production of Microorganism
Microbial Growth 1.
FERMENTATION.
Running of a pilot fermentor
Chapter 6, part A Microbial Growth.
FERMENTATION.
Chpt. 12: Respiration. Two types of respiration External Respiration Internal respiration -is the process by which - is the controlled organisms exchange.
Lecture 1: Kinetics of Substrate Utilization and Product Formation
Batch Growth Kinetics Heat generation by microbial growth
Continuous & Batch Fermentation
Title: The growth curve Homework: complete learning package 1214 January 2016.
FST 508 FERMENTED FOODS (3 units) By Dr. Olusegun Obadina.
Miss : SALSABEEL H. AL JOUJOU
Immobilized Cell System
Presented by: Shehneela Baseer Zainab Sajjad
Lec # 8 Fermentation biotechnology
DIFFERENTIATE BETWEEN PLANT, ANIMAL AND MICROBIAL BIOREACTOR
Types of Bioreactors / Fermenters
Metabolites – primary and secondary. Learning Outcomes Compare and contrast the processes of continuous culture and batch culture. Describe the differences.
Fermentation Technology
ERT314 BIOREACTOR SYSTEM CHAPTER 3: TYPES OF BIOREACTOR.
Raffia Siddique National University of Sciences and Technology sector H-12 Islamabad
Bioreactors.
Industrial Biotechnology First generation processes  Fermentation based processes using ‘native’ organisms Wine- and beer-making Baking Second generation.
Sterilization and Bioreactor Operation
TYPES OF FERMENTATION.
Batch and Continuous Flow
Scale of Fermentation process:- Large Scale, Small Scale & Pilot Scale Fermentation Presented by: Neha Agrawal m.sc. 3rd sem.
PRODUCTION OF PENICILLIN
Introduction to Fermentation
Chapter 6, part A Microbial Growth.
MIXING AND AERATION (SCALE-UP)
Optimal Reactor Configuration for Lactic Acid Production
INDUSTRIAL MICROBIOLOGY Dr. TERESA FERNÁNDEZ ALDAMA
Micro-organisms understand the role of yeast in the production of beer
Industrial Production of Citric Acid
Prof. Karkaz M. Thalij. PhD
MIC 303 INDUSTRIAL AND ENVIRONMENTAL MICROBIOLOGY
Chapter 6, part A Microbial Growth.
Enzymes in Germination
Growth & Metabolism of Micro-organisms
Bioreactors Engineering
Basis Operations in Industrial Fermentations
Chemical Engineering Explained
Biotechnology in North Carolina Today
4. Basis Operations in Food Fermentations (Biotechnology)
Lokmangal Science & Entrepreneurship College, Wadala
Metabolism and Survival
Marine Biotechnology Lab
Chapter 6, part A Microbial Growth.
Presentation transcript:

Large-Scale Production of Recombinant Proteins Lecture 6 ISAT 451, Fall 2003 College of Integrated Science and Technology James Madison University

Large-Scale Protein Production Most commercially valuable proteins (industrial enzymes or therapeutic proteins) require living cells to make the product Although cells can be readily grown in a standard 200 ml laboratory flask, commercial production may require containers in excess of 10,000 liters What are the limitations of large-scale production?

Microbial Growth Conditions Sterile culture medium containing a carbon source and essential salts or an enriched medium Pure culture for inoculation oxygenation and mixing Regulation of temperature Regulation of pH

Microbial Cell Growth

Batch Fermentation Sterile medium is inoculated and fermentation proceeds without the addition of fresh growth medium.

Fed Batch Nutrients are added at intervals during fermentation. No growth medium is removed.

Continuous Fermentation Fresh growth medium is added continuously during fermentation and spent medium is removed. Fermentation can last up to 1,000 hours.

Why do Cells Stop Growing? Depletion of nutrients Lack of oxygen Change in pH Growth inhibition from metabolic end products

Stirred Tank Bioreactors An external motor is used to agitate the growth medium with impellers. Sterile air or combinations of gases can be introduced (sparging)

Bubble Column Bioreactor Introduction of sterile air or pure gases is used for agitation and oxygenation.

Internal-Loop Airlift Bioreactor Sterile air or pure gases are introduced to create circulation internal to the reactor.

External-Loop Airlift Bioreactor Sterile air or pure gases are introduced to drive circulation through an external loop.