Multi-site recordings in Tg4510 mice

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Multi-site recordings in Tg4510 mice Liam Scott

7 month male Tg4510 mice show decreased MWM performance and hippocampal cell loss 3 vs. 7 month old Tg4510 male mice show: Area (mm2) Hippocampus: Area (Wildtype for comparison) 3m 7m Data: Yabe, Moore, Roof Data: Kawabe, Klein, Milici

Stimulation of the nPO drives the hippocampal EEG in anesthetized rats and mice 6-seconds NPO Hippo EEG Stimulus 5-HT Hippocampal Formation pcells Median Raphe Glu gaba ACh Medial Septum Nucleus Pontis Oralis (nPO) Supramammilary Bodies (SUM) Adapted from: RP Vertes (2005) Hippocampus 15:923-35 = + = - 0.06 0.08 0.10 0.12 0.14 0.16 5.5 6.0 6.5 7.0 7.5 8.0 Frequency (Hz) Stim Current (mA) Theta Frequency 0.06 0.08 0.10 0.12 0.14 0.16 15.0k 20.0k 25.0k 30.0k 35.0k 40.0k 45.0k 50.0k Theta Power Theta Power (V2) Stim Current (mA)

Determining electrode position using stimulation of nPO

7 month male Tg4510 mice show decreased nPO-evoked hippocampal theta power and total power Peak Frequency Relative Theta Power Theta Power Total Power ** * (*=P<0.05, **=P<0.01, ***=P<0.001 ; unpaired t-test)

Perforant path – dentate gyrus LTP

Determining electrode position using stimulation of perforant path

7 month male Tg4510 mice show reduced amplitude of perforant path-evoked dentate gyrus population spike

7 month male Tg4510 mice show reduced amplitude of perforant path-evoked dentate gyrus population spike

7 month male Tg4510 mice show no change in perforant path - dentate gyrus LTP

control CA-1 DG

Tg CA-1 CA1 population spike DG DG population spike

Summary Stimulated theta No change in theta frequency or relative theta Reduction in theta power and total power PP-DG LTP Reduction in amplitude of evoked population spike No change in LTP due to HFS All observations can be explained by reduction in cell number in hippocampal formation???