Journal of Allergy and Clinical Immunology

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Yvan Boutin, PhD, Jacques Hébert, MD

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Journal of Allergy and Clinical Immunology Correction Journal of Allergy and Clinical Immunology Volume 102, Issue 1, (July 1998) DOI: 10.1016/S0091-6749(98)70076-4 Copyright © 1998 Terms and Conditions

Fig. 3 Immunoblotting to ascertain presence of IgG anti-FcϵRIα. Soluble recombinant FCϵRIα was subjected to sodium dodecylsulfate–polyacrylamide gel electrophoresis and blotted onto nitrocellulose. Membranes were reacted with monoclonal antibody or whole sera from patients with chronic urticaria or normal control subjects. Antibody binding was detected with alkaline phosphatase–conjugated goat anti-mouse IgG antibody or goat anti-human IgG fragment–specific antibody, respectively. Lane 1, Molecular marker; lane 2, monoclonal antibody; lane 3, patient serum at 1:50 dilution; lane 4, serum at 1:500 dilution; lane 5, serum at 1:1000 dilution. Journal of Allergy and Clinical Immunology 1998 102, DOI: (10.1016/S0091-6749(98)70076-4) Copyright © 1998 Terms and Conditions