attP region and attB regions

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Presentation transcript:

attP region and attB regions Yordanos Teferra

attP region and attB regions Yordanos Teferra

Article 1 - "M. smegmatis involves an integrase-mediated site- Hatful, Graham. "Characterization of the Mycobacteriophage L5 Attachment Site, attp." - attP sites are located in intergenic region around an integrase gene. An integrase gene needs to be present to have an attP region within a phage and to have site-specific recombination event between attP site and attB site - "M. smegmatis involves an integrase-mediated site- specific recombination event between specific attachment sites on the phage genome (attP) and on the bacterial chromosome (attB;)"

Article 2 - Bryan Julien "Characterization of the Integrase Gene and Attachment Site for the Myxococcus xanthusBacteriophage Mx9" - attB region need to be only one in a bacteria and mostly have tRNA gene that they are located in. These region usually are the same sequence as the phage (attP) that is attaching into the bacteria. "Both phages contain attP within the int gene and integrate within a tRNA gene."

Experiment

attB

Importance These findings add on to the idea of where attP sites and attB sites are located.

Article 1 - "M. smegmatis involves an integrase-mediated site- Hatful, Graham. "Characterization of the Mycobacteriophage L5 Attachment Site, attp." - attP sites are located in intergenic region around an integrase gene. An integrase gene needs to be present to have an attP region within a phage and to have site-specific recombination event between attP site and attB site - "M. smegmatis involves an integrase-mediated site- specific recombination event between specific attachment sites on the phage genome (attP) and on the bacterial chromosome (attB;)"

Article 2 - Bryan Julien "Characterization of the Integrase Gene and Attachment Site for the Myxococcus xanthusBacteriophage Mx9" - attB region need to be only one in a bacteria and mostly have tRNA gene that they are located in. These region usually are the same sequence as the phage (attP) that is attaching into the bacteria. "Both phages contain attP within the int gene and integrate within a tRNA gene."

Experiment

attB

Importance These findings add on to the idea of where attP sites and attB sites are located.