Figure S4. A 30 32 34 36 38 40 42 44 46 48 50 time (min) 0.000 0.010 0.020 0.030 0.040 0.050 0.060 0.070 0.080 0.090 ABS (AU) at 520 nm PAP1 +AOMT Mv3R Mv3G Dp3R a3 PAP1 +AOMT::GFP Mv3R B Mv3G Dp3R a3 Figure S4. Anthocyanin profiles of transient transformed tobacco co-expressing PAP1 and AOMT (A), or co-expressing PAP1 and AOMT::GFP (B). The tobacco leaf sectors (200 mg FW) were excised 96 h after Agrobacterium-mediated transformation. Anthocyanin contents were analysed using HPLC-DAD. Anthocyanins were monitored at 520 nm. The data shown are representative of three independent experiments. Key: Dp3R, delphinidin 3-O-rutinoside; Mv3G, malvidin 3-O-glucoside; Mv3R, malvidin 3-O-rutinoside. Peak a3, malvidin 3-O-rutinoside O-hexoside.