Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction O. Kupferschmidt, D. Krüger, T.K. Held, H. Ellerhrok,

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Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction O. Kupferschmidt, D. Krüger, T.K. Held, H. Ellerhrok, W. Siegert, K. Janitschke Clinical Microbiology and Infection Volume 7, Issue 3, Pages 120-124 (March 2001) DOI: 10.1046/j.1469-0691.2001.00224.x Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Standard curve for a serial 10-fold dilution of T. gondii tachyzoites in human EDTA-blood. Each real-time PCR was performed fourfold and is indicated as mean ± standard deviation. The graph was calculated following the linear regression indicated. Clinical Microbiology and Infection 2001 7, 120-124DOI: (10.1046/j.1469-0691.2001.00224.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Gel eletrophoretic detection of the 88 bp Toxoplasma-specfic PCR products amplified by the conventional semi-nested assay. Number of tachyzoites per ml EDTA-blood: lanes 1–4:104, 10c, 10b and 10, corresponding to 2000,200,20 and two tachyzoites per 200 μL sample required for the DNA extraction kit; lane 5: negative control (EDTA-blood without tachyzoites); lane 6: standard ladder indicating 50 and 100–500 bp; lane 7: 88 bp product from a previous amplification. Clinical Microbiology and Infection 2001 7, 120-124DOI: (10.1046/j.1469-0691.2001.00224.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 Monitoring of parasitic load in the blood of an immunocompromised patient after BMT before and during Toxoplasma-specific therapy. The duration of therapy is indicated by the arrow. Clinical Microbiology and Infection 2001 7, 120-124DOI: (10.1046/j.1469-0691.2001.00224.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions