Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR A. Heininger,

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Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR A. Heininger, M. Binder, A. Ellinger, J. Pfisterer, K. Botzenhart, K. Unertl, G. Döering Clinical Microbiology and Infection Volume 10, Issue 5, Pages 452-458 (May 2004) DOI: 10.1111/j.1469-0691.2004.00877.x Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Proportion of single positive PCR results after inoculation of sterile blood with S. aureus (white bars) or E. coli (black bars) with the basic (a) and improved (b) extraction procedure. Clinical Microbiology and Infection 2004 10, 452-458DOI: (10.1111/j.1469-0691.2004.00877.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Amplification of serial dilutions of E. coli DNA in the presence and absence of DNA extracted from sterile blood samples. Lane 0: reagent negative control. Lanes 1–4: E. coli DNA (5 × 106 fg, 5 × 104 fg, 5 × 102 fg, and 5 × 10° fg) plus 5 μL of blood sample extract. Lanes 5–8: E. coli DNA (5 × 106 fg, 5 × 104 fg, 5 × 102 fg, and 5 × 10° fg) plus 5 μL of sterile water. M, DNA size marker. Clinical Microbiology and Infection 2004 10, 452-458DOI: (10.1111/j.1469-0691.2004.00877.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Results of six amplification reactions for ten sterile blood samples and varying classification thresholds for the diagnosis of bacteraemia. Clinical Microbiology and Infection 2004 10, 452-458DOI: (10.1111/j.1469-0691.2004.00877.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions