2D PAGE/Western blotting analysis for the identification of possible post-translation modification of Hsp27 from the spinal cord tissue of the SCI rat.

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2D PAGE/Western blotting analysis for the identification of possible post-translation modification of Hsp27 from the spinal cord tissue of the SCI rat. 2D PAGE/Western blotting analysis for the identification of possible post-translation modification of Hsp27 from the spinal cord tissue of the SCI rat. Protein extracts from the spinal cords of sham (A) and SCI rats not treated (B) or treated with aFGF (C) were analyzed by 2D PAGE followed by transfer onto PVDF membrane. Western blotting was performed using antibody raised against Hsp27. The isoforms of Hsp27 protein have been circled, and the molecular masses and pI values of the various protein isoforms are shown. Note that the corresponding isoforms of Hsp27 protein identified in this experiment are identical to protein spots 46 and 49 in Fig. 3. Ming-Chu Tsai et al. Mol Cell Proteomics 2008;7:1668-1687 © 2008 The American Society for Biochemistry and Molecular Biology